Recombinant Mouse Il5ra protein, His-tagged

• Cat.No.: Il5ra-5743M
• Product Overview: Recombinant Mouse Il5ra protein(P21183)(Asp18-His339), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: His
• Protein Length: Asp18-His339
• Tag: C-His
• Form: Supplied as 0.22μm filtered solution in 20mM Tris, 500mM NaCl (pH 7.4).
• Bio-activity: Mouse IL-5 R alpha, His Tag immobilized on CM5 Chip can bind Mouse IL-5, His Tag with an affinity constant of 0.495 uM as determined in SPR assay (Biacore T200).
• Molecular Mass: The protein has a predicted MW of 37.7 kDa. Due to glycosylation, the protein migrates to 50-60 kDa based on Tris-Bis PAGE result.
• Endotoxin: Less than 1EU per μg by the LAL method.
• Purity: > 95% as determined by Tris-Bis PAGE;> 95% as determined by HPLC
• Storage: Valid for 12 months from date of receipt when stored at -80°C. Recommend to aliquot the protein into smaller quantities for optimal storage. Please do not repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il5ra interleukin 5 receptor, alpha [ Mus musculus ]
• Official Symbol: Il5ra
• Synonyms: IL5RA; interleukin 5 receptor, alpha; interleukin-5 receptor subunit alpha; IL-5RA; IL-5R-alpha; IL-5R subunit alpha; IL-5 receptor alpha chain; IL-5 receptor subunit alpha; Il5r; CD125; CDw125
• Gene ID: 16192
• mRNA Refseq: NM_008370
• Protein Refseq: NP_032396