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Recombinant Mouse Interleukin 15

Cat.No. : Il15-69M
Product Overview : Recombinant Mouse Interleukin-15 produced inE.Coliis a single, non-glycosylated polypeptide chain containing 115 amino acids and having a molecular mass of 13321 Dalton. The IL-15 is purified by proprietary chromatographic techniques.
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  • Gene Information
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Cat. No. : Il15-69M
Description : The protein encoded by this gene is a cytokine that regulates T and natural killer cell activation and proliferation. This cytokine and interleukine 2 share many biological activities. They are found to bind common hematopoietin receptor subunits, and may compete for the same receptor, and thus negatively regulate each other"s activity. The number of CD8+ memory cells is shown to be controlled by a balance between this cytokine and IL2. This cytokine induces the activation of JAK kinases, as well as the phosphorylation and activation of transcription activators STAT3, STAT5, and STAT6. Studies of the mouse counterpart suggested that this cytokine may increase the expression of apoptosis inhibitor BCL2L1/BCL-x(L), possibly through the transcription activation activity of STAT6, and thus prevent apoptosis. Two alternatively spliced transcript variants of this gene encoding the same protein have been reported.
Source : Escherichia Coli.
Amino Acid Sequence : The sequence of the first five N-terminal amino acids was determined and was found to be Met-Asn-Trp-Ile-Asp.
Physical Appearance : Sterile Filtered White lyophilized (freeze-dried) powder.
Purity : Greater than 98% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
Formulation : The protein was lyophilized from a concentrated (1mg/ml) solution with 10mM NaP pH 8.0.
Solubility : It is recommended to reconstitute the lyophilized Interleukin-15 in sterile 18MΩ-cm H2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Biological Activity : The ED50as determined by the dose-dependant stimulation of the proliferation of mouse CTLL-2 was found to be 15 ng/ml, corresponding to a Specific Activity of 2 x 10⁵ IU/mg.
Storage : Lyophilized Interleukin-15 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL15 should be stored at 4℃between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles
Gene Name : Il15 interleukin 15 [ Mus musculus ]
Synonyms : Il15; interleukin 15; AI503618
Gene ID : 16168
mRNA Refseq : NM_008357
Protein Refseq : NP_032383
UniProt ID : P48346
Chromosome Location : 8 C2; 8 38.0 cM
Pathway : Cytokine-cytokine receptor interaction; Jak-STAT signaling pathway
Function : cytokine activity; cytokine receptor binding

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (10)

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What techniques differentiate IL15 and IL2 receptor binding kinetics? 10/22/2022

Techniques discern IL15 and IL2 receptor binding kinetics.

What experimental methods study IL15's involvement in autoimmune diseases? 06/23/2022

Methods explore IL15's role in autoimmune disease mechanisms.

What are the applications of IL15 knockout models in deciphering its functions? 04/26/2022

IL15 knockout models provide insights into its diverse functions.

How do you analyze IL15's impact on memory T cell differentiation? 09/19/2021

IL15's influence on memory T cell differentiation investigated.

How does immunofluorescence microscopy visualize IL15 localization in cells? 07/26/2021

Immunofluorescence microscopy visualizes IL15 subcellular localization.

What approaches validate IL15's role in inflammatory responses? 02/04/2021

Approaches validate IL15's involvement in inflammatory responses.

Could you explain the role of IL15 in NK cell cytotoxicity assays? 10/26/2019

IL15's role in NK cell cytotoxicity analyzed using assays.

Can you detail the use of ELISA in quantifying IL15 secretion? 02/03/2018

ELISA measures IL15 secretion accurately.

How is IL15's effect on regulatory T cells quantified using qPCR? 06/08/2017

qPCR quantifies IL15's impact on regulatory T cells.

How does flow cytometry assess IL15-induced T-cell proliferation? 05/26/2017

Flow cytometry quantifies IL15-driven T-cell proliferation.

Customer Reviews (3)

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Reviews
04/19/2018

    Mapping protein- interactions in DNA transcription for gene regulation.

    02/20/2018

      Suitable for cytotoxicity assays.

      10/22/2017

        Effective in NK cell activation.

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