Recombinant Mouse Kdm1a Protein, Myc/DDK-tagged
Cat.No. : | Kdm1a-3680M |
Product Overview : | Purified recombinant protein of mouse full-length lysine (K)-specific demethylase 1A (Kdm1a), with C-terminal MYC/DDK tag, expressed in HEK293T cells. |
- Specification
- Gene Information
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Description : | Histone demethylase that can demethylate both 'Lys-4' (H3K4me) and 'Lys-9' (H3K9me) of histone H3, thereby acting as a coactivator or a corepressor, depending on the context. Acts by oxidizing the substrate by FAD to generate the corresponding imine that is subsequently hydrolyzed. Acts as a corepressor by mediating demethylation of H3K4me, a specific tag for epigenetic transcriptional activation. Demethylates both mono- (H3K4me1) and di-methylated (H3K4me2) H3K4me. May play a role in the repression of neuronal genes. Alone, it is unable to demethylate H3K4me on nucleosomes and requires the presence of RCOR1/CoREST to achieve such activity. Also acts as a coactivator of androgen receptor (ANDR)-dependent transcription, by being recruited to ANDR target genes and mediating demethylation of H3K9me, a specific tag for epigenetic transcriptional repression. The presence of PRKCB in ANDR-containing complexes, which mediates phosphorylation of 'Thr-6' of histone H3 (H3T6ph), a specific tag that prevents demethylation H3K4me, prevents H3K4me demethylase activity of KDM1A. Demethylates di-methylated 'Lys-370' of p53/TP53 which prevents interaction of p53/TP53 with TP53BP1 and represses p53/TP53-mediated transcriptional activation. Demethylates and stabilizes the DNA methylase DNMT1. Required for gastrulation during embryogenesis. Component of a RCOR/GFI/KDM1A/HDAC complex that suppresses, via histone deacetylase (HDAC) recruitment, a number of genes implicated in multilineage blood cell development. Effector of SNAI1-mediated transcription repression of E-cadherin/CDH1, CDN7 and KRT8. Required for the maintenance of the silenced state of the SNAI1 target genes E-cadherin/CDH1 and CDN7. |
Source : | HEK293T |
Species : | Mouse |
Tag : | Myc/DDK |
Molecular Mass : | 93.3 kDa |
Purity : | > 80% as determined by SDS-PAGE and Coomassie blue staining |
Stability : | Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles. |
Storage : | Store at -80 centigrade after receiving vials. |
Concentration : | >50 μg/mL as determined by microplate BCA method |
Storage Buffer : | 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol. |
Gene Name : | Kdm1a lysine (K)-specific demethylase 1A [ Mus musculus (house mouse) ] |
Official Symbol : | Kdm1a |
Synonyms : | KDM1A; lysine (K)-specific demethylase 1A; lysine-specific histone demethylase 1A; lysine (K)-specific demethylase 1; BRAF35-HDAC complex protein BHC110; lysine-specific histone demethylase 1; amine oxidase (flavin containing) domain 2; flavin-containing amine oxidase domain-containing protein 2; Aof2; Kdm1; Lsd1; AA408884; mKIAA0601; D4Ertd478e; 1810043O07Rik |
Gene ID : | 99982 |
mRNA Refseq : | NM_133872 |
Protein Refseq : | NP_598633 |
UniProt ID : | Q6ZQ88 |
Products Types
◆ Recombinant Protein | ||
KDM1A-1240H | Recombinant Human KDM1A Protein, His (Fc)-Avi-tagged | +Inquiry |
KDM1A-4775M | Recombinant Mouse KDM1A Protein, His (Fc)-Avi-tagged | +Inquiry |
KDM1A-2201R | Recombinant Rhesus Macaque KDM1A Protein, His (Fc)-Avi-tagged | +Inquiry |
KDM1A-350H | Recombinant Human KDM1A Protein, MYC/DDK-tagged | +Inquiry |
KDM1A-8606H | Recombinant Human KDM1A protein, His&GST-tagged | +Inquiry |
◆ Lysates | ||
KDM1A-001HCL | Recombinant Human KDM1A cell lysate | +Inquiry |
Related Gene
For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.
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