Active Recombinant Mouse Lgals7 protein, His-tagged

Cat.No. : Lgals7-426M
Product Overview : Active Recombinant Mouse Lgals7 protein(Q99ML7)(2~136 aa), fused with N-terminal His tag, was expressed in E.coli.
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Species : Mouse
Source : E.coli
Tag : His
Protein Length : 2-136 aa
Form : Lyophilized from sterile PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Bio-activity : The galectins constitute a large family of carbohydrate-binding proteins with specificity for N-acetyl-lactosamine-containing glycoproteins. At least 14 mammalian galectins, which share structural similarities in their carbohydrate recognition domains (CRD), have been identified. The galectins have been classified into the prototype galectins (-1, -2, -5, -7, -10, -11, -13, -14), which contain one CRD and exist either as a monomer or a noncovalent homodimer; the chimera galectins (Galectin-3) containing one CRD linked to a nonlectin domain; and the tandem-repeat galectins (-4, -6, -8, -9, -12) consisting of two CRDs joined by a linker peptide. Galectin-7 may also be involved in cell-cell and cell-matrix interactions and exogenous galectin has been found to accelerate the re-epithelialization of wounds It can agglutinate red blood.In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant Mu GAL7 with 0.9% sodium chloride injection, add 50μL a serial dilution of GAL7 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control with no GAL7, replace with 50μL 0.9% sodium chloride injection. Then add 50μL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown. It was obvious that the minimal effective concentration of GAL7 is 3.125 μg/mL.
Molecular Mass : 18.9kDa
Endotoxin : <1.0EU per 1μg (determined by the LAL method).
Purity : > 97% as determined by SDS-PAGE.
Storage : Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name Lgals7 lectin, galactose binding, soluble 7 [ Mus musculus ]
Official Symbol Lgals7
Synonyms LGALS7; lectin, galactose binding, soluble 7; galectin-7; gal-7; Pig1; Galectin-7; MGC151215; MGC151217;
Gene ID 16858
mRNA Refseq NM_008496
Protein Refseq NP_032522

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