Recombinant Mouse Map2k2 Protein, Myc/DDK-tagged

• Cat.No.: Map2k2-3921M
• Product Overview: Purified recombinant protein of mouse full-length mitogen-activated protein kinase kinase 2 (Map2k2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Catalyzes the concomitant phosphorylation of a threonine and a tyrosine residue in a Thr-Glu-Tyr sequence located in MAP kinases. Activates the ERK1 and ERK2 MAP kinases. Activates BRAF in a KSR1 or KSR2-dependent manner; by binding to KSR1 or KSR2 releases the inhibitory intramolecular interaction between KSR1 or KSR2 protein kinase and N-terminal domains which promotes KSR1 or KSR2-BRAF dimerization and BRAF activation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 44.9 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Map2k2 mitogen-activated protein kinase kinase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Map2k2
• Synonyms: MAP2K2; mitogen-activated protein kinase kinase 2; dual specificity mitogen-activated protein kinase kinase 2; MEK 2; MAPKK 2; MAPK/ERK kinase 2; MAP kinase kinase 2; MAP kinase/Erk kinase; ERK activator kinase 2; mitogen activated protein kinase kinase 2; protein kinase, mitogen activated, kinase 2, p45; MK2; MEK2; Prkmk2; AA589381
• Gene ID: 26396
• mRNA Refseq: NM_023138
• Protein Refseq: NP_075627
• UniProt ID: Q63932