Recombinant Mouse MAP2K4 Protein (2-397 aa), His-SUMO-tagged

• Cat.No.: MAP2K4-641M
• Product Overview: Recombinant Mouse MAP2K4 Protein (2-397 aa) is produced by E. coli expression system. This protein is fused with a 6xHis-SUMO tag at the N-terminal. Protein Description: Full Length of Mature Protein.

Specification

• Description: Dual specificity protein kinase which acts as an essential component of the MAP kinase signal transduction pathway. Essential component of the stress-activated protein kinase/c-Jun N-terminal kinase (SAP/JNK) signaling pathway. With MAP2K7/MKK7, is the one of the only known kinase to directly activate the stress-activated protein kinase/c-Jun N-terminal kinases MAPK8/JNK1, MAPK9/JNK2 and MAPK10/JNK3. MAP2K4/MKK4 and MAP2K7/MKK7 both activate the JNKs by phosphorylation, but they differ in their preference for the phosphorylation site in the Thr-Pro-Tyr motif. MAP2K4 shows preference for phosphorylation of the Tyr residue and MAP2K7/MKK7 for the Thr residue. The phosphorylation of the Thr residue by MAP2K7/MKK7 ses to be the prerequisite for JNK activation at least in response to proinflammatory cytokines, while other stimuli activate both MAP2K4/MKK4 and MAP2K7/MKK7 which synergistically phosphorylate JNKs. MAP2K4 is required for maintaining peripheral lymphoid homeostasis. The MKK/JNK signaling pathway is also involved in mitochondrial death signaling pathway, including the release cytochrome c, leading to apoptosis. Whereas MAP2K7/MKK7 exclusively activates JNKs, MAP2K4/MKK4 additionally activates the p38 MAPKs MAPK11, MAPK12, MAPK13 and MAPK14.
• Source: E. coli
• Species: Mouse
• Tag: His-SUMO
• Form: Tris-based buffer, 50% glycerol
• Molecular Mass: 60.0 kDa
• Protein length: 2-397 aa
• AA Sequence: AAPSPSGGGGSGGGGGTPGPIGPPA SGHPAVSSMQGKRKALKLNFANPPV KSTARFTLNPNTTGVQNPHIERLRT HSIESSGKLKISPEQHWDFTAEDLK DLGEIGRGAYGSVNKMVHKPSGQIM AVKRIRSTVDEKEQKQLLMDLDVVM RSSDCPYIVQFYGALFREGDCWICM ELMSTSFDKFYKYVYSVLDDVIPEE ILGKITLATVKALNHLKENLKIIHR DIKPSNILLDRSGNIKLCDFGISGQ LVDSIAKTRDAGCRPYMAPERIDPS ASRQGYDVRSDVWSLGITLYELATG RFPYPKWNSVFDQLTQVVKGDPPQL SNSEEREFSPSFINFVNLCLTKDES KRPKYKELLKHPFILMYEERTVEVA CYVCKILDQMPATPSSPMYVD
• Purity: > 90% as determined by SDS-PAGE.
• Notes: Repeated freezing and thawing is not recommended. Store working aliquots at 4 centigrade for up to one week.
• Storage: The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself. Generally, the shelf life of liquid form is 6 months at -20 centigrade/-80 centigrade. The shelf life of lyophilized form is 12 months at -20 centigrade/-80 centigrade.
• Concentration: A hardcopy of COA with concentration instruction is sent along with the products.

Gene Information

• Gene Name: Map2k4 mitogen-activated protein kinase kinase 4 [ Mus musculus ]
• Official Symbol: MAP2K4
• Synonyms: MAP2K4; MEK 4; JNKK 1; MAPKK 4; JNK kinase 1; MEK4; MKK4; Sek1; JNKK1; Serk1; PRKMK4
• Gene ID: 26398
• mRNA Refseq: NM_009157
• Protein Refseq: NP_033183
• UniProt ID: P47809