Recombinant Mouse Misp Protein, Myc/DDK-tagged

• Cat.No.: Misp-4080M
• Product Overview: Purified recombinant protein of mouse full-length mitotic spindle positioning (Misp), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Species: Mouse
• Source: HEK293
• Tag: DDK&Myc
• Description: Plays a role in mitotic spindle orientation and mitotic progression. Regulates the distribution of dynactin at the cell cortex in a PLK1-dependent manner, thus stabilizing cortical and astral microtubule attachments required for proper mitotic spindle positioning. May link microtubules to the actin cytoskeleton and focal adhesions. May be required for directed cell migration and centrosome orientation. May also be necessary for proper stacking of the Golgi apparatus.
• Molecular Mass: 72.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Misp mitotic spindle positioning [ Mus musculus (house mouse) ]
• Official Symbol: Misp
• Synonyms: Misp; mitotic spindle positioning; 9130017N09Rik; mitotic interactor and substrate of PLK1; mitotic spindle positioning protein
• Gene ID: 78906
• mRNA Refseq: NM_030218
• Protein Refseq: NP_084494
• UniProt ID: Q9D279