Recombinant Mouse Mmp2, His-tagged

• Cat.No.: Mmp2-416M
• Product Overview: A DNA sequence encoding the mouse Mmp2 (NP_032636.1) (Met409-Cys622) was expressed with a polyhistidine tag at the N-terminus.

Specification

• Description: 72 kDa type IV collagenase also known as matrix metalloproteinase-2 (MMP-2) and gelatinase A is an enzyme that in humans is encoded by the MMP2 gene. Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. This gene encodes an enzyme which degrades type IV collagen, the major structural component of basement membranes. The enzyme plays a role in endometrial menstrual breakdown, regulation of vascularization and the inflammatory response.
• Source: Human Cells
• Species: Mouse
• Tag: His
• Predicted N Terminal: His
• Form: Lyophilized from sterile PBS, pH 7.4.
• Molecular Mass: The recombinant mouse Mmp2 consists 273 amino acids and predicts a molecular mass of 30.9 kDa.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
• Purity: > 95 % as determined by SDS-PAGE.
• Stability: Samples are stable for up to twelve months from date of receipt at -70℃
• Storage: Store it under sterile conditions at -70℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.

Gene Information

• Gene Name: Mmp2 matrix metallopeptidase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Mmp2
• Synonyms: Mmp2; GelA; Clg4a; MMP-2; 72 kDa type IV collagenase; gelatinase A; 72kD gelatinase; 72kDa gelatinase; 72 kDa gelatinase; 72kD type IV collagenase; 72kDa type IV collagenase; matrix metalloproteinase 2; matrix metalloproteinase-2; NP_032636.1; EC 3.4.24.24
• Gene ID: 17390
• mRNA Refseq: NM_008610
• Protein Refseq: NP_032636
• UniProt ID: P33434
• Chromosome Location: 8 C5; 8 44.99 cM
• Pathway: Activation of Matrix Metalloproteinases; Collagen degradation; Estrogen signaling pathway
• Function: fibronectin binding; hydrolase activity; metal ion binding

Reviews

06/19/2021

The solubility and ion exchange ability are very good, which is convenient for subsequent experimental treatment and purification.

05/13/2021

Kinetic parameters such as enzymatic reaction rate and binding affinity were tested in the early stage, and it was found that the protein performed well.

03/16/2021

MMP2 products undergo strict quality control to ensure the consistency and stability of each batch.

Q&As

How to regulate the activity of MMP2 in tumor therapy?

The activity of MMP2 is regulated by inhibitors and antibodies against it to inhibit tumor metastasis and invasion.

How is MMP2 related to metastases that promote disease progression?

MMP2 interacts with some transfer factors, such as VEGF and EGF, to promote tumor metastasis and invasion.

What are the application prospects of MMP2 inhibitors?

Inhibitors of MMP2 can be used as potential drug targets for the treatment of tumors, cardiovascular diseases and other related diseases.

Does MMP2 interact with the tumor microenvironment?

MMP2 interacts with tumor cells, immune cells, blood vessels, etc. in the tumor microenvironment to affect tumor progression and treatment effect.

What is the role of MMP2 in embryonic development?

It is involved in important processes such as morphogenesis, organ development and intraembryonic lymphatic vessel formation during embryonic development.

What are the effects of MMP2 mutations in human disease?

Mutations in MMP2 may affect its protein structure and function, leading to the occurrence and progression of related diseases.