| Species : |
Mouse |
| Source : |
HEK293 |
| Tag : |
His |
| Protein Length : |
2-305 aa |
| Description : |
The protein encoded by this gene is a DNA binding homeobox transcription factor involved in embryonic stem (ES) cell proliferation, renewal, and pluripotency. The encoded protein can block ES cell differentiation and can also autorepress its own expression in differentiating cells. Several transcript variants encoding different isoforms have been found for this gene. |
| Form : |
Pink liquid, Culture supernatant of transfected HEK293 cells |
| Bio-activity : |
Measured by its binding ability in a transcription factor ELISA. Immobilized DNA containing the Nanog consensus binding sequence (TAATGG) can bind human Nanog with an EC50 of 24.41 AU/mL. |
| Molecular Mass : |
34 kDa |
| Purity : |
Unpurified cell culture supernatant. HEK293 cells grown in serum-free medium were transfected with expression vector harboring target gene. The cell culture was harvested with centrifugation to remove cells. The cell culture supernatant containing mammalian cell protease inhibitor cocktail was aliquoted and stored at -80 centigrade immediately. The gene overexpression in culture supernatant was confirmed by Western blotting using anti-His tag antibody and/or target-specific antibodies and the culture supernatant derived from HEK293 cells transfected with the empty expression vector was used as a negative control. |
| Storage : |
Upon arrival, the protein may be stored for 2 weeks at 4 centigrade. For long term storage, it is recommended to store at -20 centigrade or -80 centigrade in appropriate aliquots. Avoid repeated freeze-thaw cycles. |
| Shipping : |
Blue ice |
