Active Recombinant Mouse nonagouti Protein

• Cat.No.: nonagouti-01M
• Product Overview: Recombinant Mouse ASIP/Agouti Protein (His23-Cys131) without tag was expressed in Mouse myeloma cell line.

Specification

• Description: Involved in the regulation of melanogenesis. The binding of ASP to MC1R precludes alpha-MSH initiated signaling and thus blocks production of cAMP, leading to a down-regulation of eumelanogenesis (brown/black pigment) and thus increasing synthesis of pheomelanin (yellow/red pigment). Causes hair follicle melanocytes to synthesize phaeomelanin instead of black or brown pigment eumelanin and produces hairs with a subapical yellow band on an otherwise black or brown background when expressed during the mid-portion of hair growth.
• Source: Mouse myeloma cell line
• Species: Mouse
• Bio-activity: Measured by its ability to inhibit alpha-MSH induced eumelanin production in B16F1 mouse melanoma cells. The ED50 for this effect is 0.8-4 μg/mL.
• Molecular Mass: 12 kDa
• N-terminal Sequence Analysis: His23
• Endotoxin: < 0.10 EU/μg of the protein by the LAL method.
• Purity: > 90% by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
• Storage: Avoid repeated freeze-thaw cycles.
  12 months from date of receipt, -20 to -70 centigrade as supplied.
  1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
  3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Storage Buffer: Lyophilized from a 0.2 μm filtered solution in PBS.
• Reconstitution: Reconstitute at 500 μg/mL in PBS.

Gene Information

• Gene Name: a nonagouti [ Mus musculus (house mouse) ]
• Official Symbol: a
• Synonyms: a; nonagouti; agouti-signaling protein; agouti coat color protein; agouti signal protein; agouti switch protein; non-agouti; As; ASP; ago; A; ASIP
• Gene ID: 50518
• mRNA Refseq: NM_015770
• Protein Refseq: NP_056585
• UniProt ID: Q03288