Recombinant Mouse Nos2 protein, His-tagged

• Cat.No.: Nos2-7799M
• Product Overview: Recombinant Mouse Nos2 aa. (Ser868~Asp909) fused with N-terminal His tag was produced in E. coli cells.

Specification

• Description: Nitric oxide is a reactive free radical which acts as a biologic mediator in several processes, including neurotransmission and antimicrobial and antitumoral activities. This gene encodes a nitric oxide synthase that is inducible by a combination of lipopolysaccharide and certain cytokines. Three transcript variants encoding two different isoforms have been found for this gene.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: 10kDa as determined by SDS-PAGE reducing conditions.
• Protein length: Ser868~Asp909
• Endotoxin: <1.0EU per 1µg (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 7.8.
• Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine Reactive Labeling
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Concentration: 200μg/mL
• Storage buffer: PBS, pH7.4, containing 1mM DTT, 5% trehalose, 0.01% sarcosyl and Proclin300
• Reconstitution: Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Nos2 nitric oxide synthase 2, inducible [ Mus musculus (house mouse) ]
• Official Symbol: Nos2
• Synonyms: Nos2; nitric oxide synthase 2, inducible; iNOS; Nos-2; Nos2a; i-NOS; NOS-II; MAC-NOS; nitric oxide synthase, inducible; NOS type II; inducible NO synthase; inducible NOS; inducible nitric oxide synthase; macrophage NOS; nitric oxide synthase 2, inducible, macrophage; peptidyl-cysteine S-nitrosylase NOS2
• Gene ID: 18126
• mRNA Refseq: NM_001313921.1
• Protein Refseq: NP_001300850.1
• UniProt ID: P29477