Recombinant Mouse Opa1 Protein, Myc/DDK-tagged

• Cat.No.: Opa1-4592M
• Product Overview: Purified recombinant protein of mouse full-length OPA1, mitochondrial dynamin like GTPase (Opa1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Dynamin-related GTPase that is essential for normal mitochondrial morphology by regulating the equilibrium between mitochondrial fusion and mitochondrial fission. Coexpression of isoform 1 with shorter alternative products is required for optimal activity in promoting mitochondrial fusion. Binds lipid membranes enriched in negatively charged phospholipids, such as cardiolipin, and promotes membrane tubulation. The intrinsic GTPase activity is low, and is strongly increased by interaction with lipid membranes. Plays a role in remodeling cristae and the release of cytochrome c during apoptosis. Proteolytic processing in response to intrinsic apoptotic signals may lead to disassembly of OPA1 oligomers and release of the caspase activator cytochrome C (CYCS) into the mitochondrial intermembrane space. Plays a role in mitochondrial genome maintenance.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 111.3 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Opa1 OPA1, mitochondrial dynamin like GTPase [ Mus musculus (house mouse) ]
• Official Symbol: Opa1
• Synonyms: OPA1; optic atrophy 1 homolog (human); dynamin-like 120 kDa protein, mitochondrial; largeG; large GTP-binding protein; optic atrophy protein 1 homolog; lilr3; AI225888; AI847218; mKIAA0567; 1200011N24Rik
• Gene ID: 74143
• mRNA Refseq: NM_133752
• Protein Refseq: NP_598513
• UniProt ID: P58281