Recombinant Mouse P4hb Protein, Myc/DDK-tagged

• Cat.No.: P4hb-4642M
• Product Overview: Purified recombinant protein of mouse full-length prolyl 4-hydroxylase, beta polypeptide (P4hb), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This multifunctional protein catalyzes the formation, breakage and rearrangement of disulfide bonds. At the cell surface, seems to act as a reductase that cleaves disulfide bonds of proteins attached to the cell. May therefore cause structural modifications of exofacial proteins. Inside the cell, seems to form/rearrange disulfide bonds of nascent proteins. At high concentrations, functions as a chaperone that inhibits aggregation of misfolded proteins. At low concentrations, facilitates aggregation (anti-chaperone activity). May be involved with other chaperones in the structural modification of the TG precursor in hormone biogenesis. Also acts a structural subunit of various enzymes such as prolyl 4-hydroxylase and microsomal triacylglycerol transfer protein MTTP. Receptor for LGALS9; the interaction retains P4HB at the cell surface of Th2 T helper cells, increasing disulfide reductase activity at the plasma membrane, altering the plasma membrane redox state and enhancing cell migration
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 57.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: P4hb prolyl 4-hydroxylase, beta polypeptide [ Mus musculus (house mouse) ]
• Official Symbol: P4hb
• Synonyms: P4HB; prolyl 4-hydroxylase, beta polypeptide; protein disulfide-isomerase; p55; ER protein 59; protein disulfide isomerase; prolyl 4-hydroxylase subunit beta; cellular thyroid hormone-binding protein; endoplasmic reticulum resident protein 59; PDI; Thbp; ERp59; Pdia1
• Gene ID: 18453
• mRNA Refseq: NM_011032
• Protein Refseq: NP_035162
• UniProt ID: P09103