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Recombinant Mouse Pcsk9 Protein, His-Avi-tagged, Biotinylated

Cat.No.: Pcsk9-209M
Product Overview: Biotinylated Mouse PCSK9 is expressed from HEK293 cells. It contains AA Gln 35 - Gln 694 (Accession # AAH38085.1). This protein carries an Avi tag at the C-terminus, followed by a polyhistidine tag.
Source: HEK293
Species: Mouse
Tag : His-Avi
Predicted N Terminal: Gln 35
Form: Lyophilized from 0.22 um filtered solution in PBS, pH7.4, 10% trehalose.
Bio-activity: Immobilized Mouse LDL R, His Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Mouse PCSK9, His Tag with a linear range of 2-16 ug/mL.
Molecular Mass: The protein has a calculated MW of 74.9 kDa. The protein migrates as 20 kDa and 65 kDa on a SDS-PAGE gel under reducing condition due to glycosylation and proteolytic digestion.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95% as determined by SDS-PAGE.
Characteristic: Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Pcsk9 proprotein convertase subtilisin/kexin type 9 [ Mus musculus ]
Official Symbol: Pcsk9
Synonyms: PCSK9; proprotein convertase subtilisin/kexin type 9; NARC-1; convertase subtilisin; proprotein convertase 9; proprotein convertase PC9; subtilisin/kexin-like protease PC9; neural apoptosis regulated convertase 1; neural apoptosis-regulated convertase 1; FH3; PC9; Narc1; HCHOLA3; AI415265; AI747682; MGC47409;
Gene ID: 100102
mRNA Refseq: NM_153565
Protein Refseq: NP_705793

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