Recombinant Mouse Pdcd1 Protein, Fc-Avi-tagged, Biotinylated

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Recombinant Mouse Pdcd1 Protein, Fc-Avi-tagged, Biotinylated

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Cat.No. : Pdcd1-206M
Product Overview : Biotinylated Mouse PD-1 / PDCD1, Fc Tag is expressed from HEK293 cells. It contains AA Leu 25 - Gln 167 (Accession # NP_032824). This protein carries a human IgG1 Fc fragment at the C-terminus, followed by an Avi tag.
Source : HEK293
Species : Mouse
Tag : Fc-Avi
Predicted N Terminal : Leu 25
Form : Lyophilized from 0.22 um filtered solution in 50 mM Tris, 100 mM Glycine, pH7.5, 10% trehalose.
Bio-activity : Immobilized Mouse PD-L1 / B7-H1 Protein, Fc Tag at 10 ug/mL (100 uL/well) can bind Biotinylated Mouse PD-1, Fc tag with a linear range of 0.15-2.5 ug/mL.
Molecular Mass : The protein has a calculated MW of 44.6 kDa. The protein migrates as 55-66 kDa on a SDS-PAGE gel under reducing condition due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >92% as determined by SDS-PAGE.
Characteristic : Biotinylation of this product is performed using Avitag technology. Briefly, the single lysine residue in the Avitag is enzymatically labeled with biotin. The biotin to protein ratio is 0.5-1 as determined by the HABA assay.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : For long term storage, the product should be stored at lyophilized state at -20ºC or lower. Please avoid repeated freeze-thaw cycles. No activity loss is observed after storage at: 4-8ºC for 12 months in lyophilized state; -70ºC for 3 months under sterile conditions after reconstitution.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : Pdcd1 programmed cell death 1 [ Mus musculus ]
Official Symbol : Pdcd1
Synonyms : PDCD1; programmed cell death 1; programmed cell death protein 1; mPD-1; protein PD-1; PD-1; Pdc1; Ly101;
Gene ID : 18566
mRNA Refseq : NM_008798
Protein Refseq : NP_032824

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