Active Recombinant Mouse Pf4 protein, hFc-tagged

• Cat.No.: Pf4-6743M
• Product Overview: Recombinant Mouse Pf4 protein(Val30-Ser105), fused with C-terminal hFc tag, was expressed in HEK293.

Specification

• Species: Mouse
• Source: HEK293
• Tag: Fc
• Protein Length: 30-105 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4).Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Measured by its binding ability in a functional ELISA. Immobilized Human CCL5 at 5 μg/mL (100 μL/well) can bind Mouse CXCL4 with a linear range of 10-252.6 ng/mL.
• Molecular Mass: 34.17 kDa
• Endotoxin: < 0.01 EU/μg of the protein by LAL method
• Purity: ≥ 90% as determined by SDS-PAGE; ≥ 90% as determined by HPLC.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Pf4 platelet factor 4 [ Mus musculus ]
• Official Symbol: Pf4
• Synonyms: PF4; platelet factor 4; PF-4; C-X-C motif chemokine 4; chemokine (C-X-C motif) ligand 4; Cxcl4; Scyb4
• Gene ID: 56744
• mRNA Refseq: NM_019932
• Protein Refseq: NP_064316