Recombinant Mouse Plb1 Protein, Myc/DDK-tagged

• Cat.No.: Plb1-4910M
• Product Overview: Purified recombinant protein of mouse full-length phospholipase B1 (Plb1), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Calcium-independent membrane-associated phospholipase that catalyzes complete diacylation of phospholipids by hydrolyzing both sn-1 and sn-2 fatty acyl chains attached to the glycerol backbone (phospholipase B activity). Has dual phospholipase and lysophospholipase activities toward diacylphospholipids. Preferentially cleaves sn-2 ester bonds over sn-1 bonds. Acts as a lipase toward glycerolipid substrates. Hydrolyzes fatty acyl chains of diacylglycerols with preference for the sn-2 position and of triacylglycerols with not positional selectivity. May also hydrolyze long chain retinyl esters such as retinyl palmitate. May contribute to digestion of dietary phospholipids, glycerolipids and retinoids, facilitating lipid absorption at the brush border.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 165 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Plb1 phospholipase B1 [ Mus musculus (house mouse) ]
• Official Symbol: Plb1
• Synonyms: Plb1; phospholipase B1; BC033606; 4632413E21Rik; 4930433E17Rik; 4930539A06Rik; phospholipase B1, membrane-associated; PLB/LIP; lysophospholipase; phospholipase A2; phospholipase B; phospholipase B/lipase; triacylglycerol lipase; EC 3.1.1.3; EC 3.1.1.4; EC 3.1.1.5
• Gene ID: 665270
• mRNA Refseq: NM_001081407
• Protein Refseq: NP_001074876
• UniProt ID: Q3TTY0