Recombinant Mouse Plcd4 Protein, Myc/DDK-tagged

• Cat.No.: Plcd4-4915M
• Product Overview: Purified recombinant protein of mouse full-length phospholipase C, delta 4 (Plcd4), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Hydrolyzes the phosphatidylinositol 4,5-bisphosphate (PIP2) to generate 2 second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3). DAG mediates the activation of protein kinase C (PKC), while IP3 releases Ca2+ from intracellular stores. Required for acrosome reaction in sperm during fertilization, probably by acting as an important enzyme for intracellular Ca2+ mobilization in the zona pellucida-induced acrosome reaction. May play a role in cell growth. Modulates the liver regeneration in cooperation with nuclear PKC. Overexpression up-regulates the Erk signaling pathway and proliferation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 92.7 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Plcd4 phospholipase C, delta 4 [ Mus musculus (house mouse) ]
• Official Symbol: Plcd4
• Synonyms: PLCD4; phospholipase C, delta 4; 1-phosphatidylinositol-4,5-bisphosphate phosphodiesterase delta-4; PLC-delta-4; phospholipase C-delta-4; phosphoinositide phospholipase C-delta-4; 4921507K24Rik
• Gene ID: 18802
• mRNA Refseq: NM_148937
• Protein Refseq: NP_683739
• UniProt ID: Q8K3R3