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Active Recombinant Mouse RSPO3 Protein

Cat.No. : RSPO3-14554M
Product Overview : Recombinant Mouse RSPO3 Protein (Met1-His277) without tag was expressed in Chinese Hamster Ovary cell line.
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Description : Activator of the canonical Wnt signaling pathway by acting as a ligand for LGR4-6 receptors, which acts as a key regulator of angiogenesis. Upon binding to LGR4-6 (LGR4, LGR5 or LGR6), LGR4-6 associate with phosphorylated LRP6 and frizzled receptors that are activated by extracellular Wnt receptors, triggering the canonical Wnt signaling pathway to increase expression of target genes. Also regulates the canonical Wnt/beta-catenin-dependent pathway and non-canonical Wnt signaling by acting as an inhibitor of ZNRF3, an important regulator of the Wnt signaling pathway. Acts as a ligand for frizzled FZD8 and LRP6. May negatively regulate the TGF-beta pathway. Acts as a key regulator of angiogenesis by controlling vascular stability and pruning: acts by activating the non-canonical Wnt signaling pathway in endothelial cells. Can also amplify Wnt signaling pathway independently of LGR4-6 receptors, possibly by acting as a direct antagonistic ligand to RNF43 and ZNRF3.
Source : CHO
Species : Mouse
Bio-activity : Measured by its ability to induce Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is 0.25-1 ng/mL in the presence of 5 ng/mL Recombinant Mouse Wnt‑3a.
Molecular Mass : 28.9 kDa
N-terminal Sequence Analysis : Met33
Endotoxin : < 0.10 EU/μg of the protein by the LAL method.
Purity : > 95%, by SDS-PAGE visualized with Silver Staining and quantitative densitometry by Coomassie® Blue Staining.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 centigrade as supplied.
1 month, 2 to 8 centigrade under sterile conditions after reconstitution.
3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Storage Buffer : Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Reconstitution : Reconstitute at 200 μg/mL in PBS.
Shipping : The product is shipped at ambient temperature.
Gene Name : Rspo3 R-spondin 3 [ Mus musculus (house mouse) ]
Official Symbol : Rspo3
Synonyms : Rspo3; R-spondin 3; Cri; Thsd; Thsd2; AW742308; Cristin1; 2810459H04Rik; R-spondin-3; R-spondin 3 homolog; cabriolet; cristin 1; cysteine-rich and single thrombospondin domain-containing protein 1; nucleopondin; roof plate-specific spondin-3; thrombospondin, type I, domain 2
Gene ID : 72780
mRNA Refseq : NM_028351
Protein Refseq : NP_082627
UniProt ID : Q2TJ95

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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Q&As (7)

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How do genetic variations in RSPO3 affect developmental processes? 10/12/2022

Genetic mutations in RSPO3 can disrupt normal development and tissue regeneration.

What potential therapeutic applications arise from targeting RSPO3 in regenerative medicine and cancer? 03/15/2021

Targeting RSPO3 could offer advances in regenerative medicine and cancer therapy by modulating Wnt signaling.

What role does RSPO3 play in stem cell biology? 05/03/2020

RSPO3 plays a significant role in maintaining stem cell populations and influencing their differentiation.

How does RSPO3 contribute to tissue development and regeneration? 04/14/2020

It stimulates tissue development and regeneration by activating Wnt pathways, essential for cellular proliferation.

What is the primary role of RSPO3 in Wnt signaling? 12/21/2018

RSPO3, a member of the R-spondin family, enhances Wnt signaling, crucial for cellular growth and differentiation.

What is the impact of altered RSPO3 activity on cancer progression? 02/15/2018

Altered RSPO3 activity is linked to cancer development and progression, particularly in colorectal cancer.

How does RSPO3 interact with other components in Wnt signaling pathways? 04/03/2017

RSPO3 interacts synergistically with Wnt pathway components, amplifying signal transduction.

Customer Reviews (3)

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07/24/2022

    Dependable partner, a vital research collaborator.

    09/09/2021

      Consistency in quality, invaluable for our experiments.

      03/22/2020

        Reliable data source, accelerates our research progress.

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