Recombinant Mouse S100 Calcium Binding Protein A5, His-tagged

• Cat.No.: S100a5-5588M
• Product Overview: Recombinant mouse S100a5protein, fused to His-tag at N-terminus, was expressed in E. coli andpurified by using conventional chromatography techniques.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Description: S100calcium binding protein A5, also known as S100a5, is a member of the S100 family of proteins containing 2EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasmand nucleus of a wide range of cells, and involved in the regulation of anumber of cellular processes such as cell cycle progression anddifferentiation. S100a5 has a Ca2+ affinity20- to 100-fold higher than the other S100 proteins studied under identicalconditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly whichimpairs the binding of Ca2+. It is expressed in very restricted regions ofthe adult brain.
• Form: Liquid. In 20 mMTris-HCl buffer, pH8.0, 40% glycerol, 3mM DTT, 200mM NaCl.
• Molecular Weight: 13.4kDa(117aa), confirmed by MALDI-TOF.
• Purity: > 95%by SDS-PAGE
• Concentration: 1 mg/ml(determined by Bradford assay)
• Sequences of aminoacids: MGSSHHHHHHSSGLVPRGSH MGSHMETPLE KALTTMVTTF HKYSGREGSK LTLSRKELKE LIKTELSLAEKMKESSIDNL MKSLDKNSDQ EIDFKEYSVF LTTLCMAYND FFLEDNK
• Storage: Can bestored at +4°C short term (1-2 weeks). For long term storage, aliquot andstore at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: S100a5 S100 calcium bindingprotein A5 [ Mus musculus ]
• Official Symbol: S100a5
• Synonyms: S100a5; S100 calcium binding protein A5;S100D9; protein S100-A5; Protein S-100D; S100 calcium-binding protein A5
• Gene ID: 20199
• mRNA Refseq: NM_011312
• Protein Refseq: NP_035442
• UniProt ID: P63084
• Chromosome Location: 3 F1-F2; 3 43.6 cM
• Function: calcium ion binding; copper ion binding;metal ion binding; protein homodimerization activity; zinc ion binding