Recombinant Human S100A5, His-tagged

• Cat.No.: S100A5-3415H
• Product Overview: Recombinant human S100A5 protien, fused to His-tag at N-terminus, was expressed in E.coli and purified by using conventional chromatography techniques.

Specification

• Species: Human
• Source: E.coli
• Tag: His
• Description: S100 calcium binding protein A5, also known as S100A5, is a member of the S100 family of proteins containing 2 EF-hand calcium-binding motifs. S100 proteins are localized in the cytoplasm and nucleus of a wide range of cells, and involved in the regulation of a number of cellular processes such as cell cycle progression and differentiation. S100A5 has a Ca2+ affinity 20- to 100-fold higher than the other S100 proteins studied under identical conditions. This protein also binds Zn2+ and Cu2+, and Cu2+ strongly which impairs the binding of Ca2+. S100A5 is expressed in very restricted regions of the adult brain.
• Form: Liquid. In 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 30% glycerol, 0.1M NaCl, 0.1mM PMSF.
• Molecular Weight: 12.9 kDa (112aa) confirmed by MALDI-TOF.
• Purity: > 90% by SDS - PAGE.
• Concentration: 1 mg/ml (determined by Bradford assay).
• Sequences Of Amino Acids: MGSSHHHHHH SSGLVPRGSH METPLEKALT TMVTTFHKYS GREGSKLTLS RKE LKELIKK ELCLGEMKES SIDDLMKSLD KNSDQEIDFKEYSVFLTMLC MAY NDF FLED NK.
• Storage: Can be stored at +4°C short term (1-2 weeks). For long term storage, aliquot and store at -20°C or -70°C. Avoid repeated freezing and thawing cycles.

Gene Information

• Gene Name: S100A5 S100 calcium binding protein A5 [ Homo sapiens ]
• Synonyms: S100A5; S100 calcium binding protein A5; S100D; S100A5; Protein S-100D; S100 calcium-binding protein A5; Protein S-100D
• Gene ID: 6276
• mRNA Refseq: NM_002962
• Protein Refseq: NP_002953
• MIM: 176991
• UniProt ID: P33763
• Chromosome Location: 1q21
• Function: calcium ion binding