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Recombinant Mouse Sell Protein, His & Fc-tagged

Cat.No. : Sell-831M
Product Overview : Recombinant extracellular domain (Met 1-Asn 332) of mouse SELL (NP_035476.1) precursor was fused with the C-terminal polyhistidine-tagged Fc region of human IgG1 at the C-terminus.
Source : HEK293
Species : Mouse
Tag : His & Fc
Predicted N Terminal : Trp 39
Form : Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity : Measured by the ability of the immobilized protein to support the adhesion of U937 human histiocytic lymphoma cells. When cells are added to SELL coated plates (10 μg/mL, 100 μL/well) approximately >60% cells will adhere specifically.
Molecular Mass : The recombinant mouse SELL/Fc is a disulfide-linked homodimer after removal of the signal peptide. The reduced monomer consists of 542 amino acids and has a predicted molecular mass of 61 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rm SELL/Fc monomer is approximately 100-110 kDa due to glycosylation.
Endotoxin : < 1.0 EU per μg of the protein as determined by the LAL method.
Purity : >90 % as determined by SDS-PAGE.
Stability : Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage : Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution : It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name : Sell selectin, lymphocyte [ Mus musculus ]
Official Symbol : Sell
Synonyms : SELL; selectin, lymphocyte; L-selectin; LAM-1; LECAM1; lymphocyte antigen 22; lymph node homing receptor; leukocyte adhesion molecule 1; CD62 antigen-like family member L; lymphocyte surface MEL-14 antigen; leukocyte-endothelial cell adhesion molecule 1;
Gene ID : 20343
mRNA Refseq : NM_001164059
Protein Refseq : NP_001157531
MIM :
UniProt ID :
Pathway : Adaptive Immune System, organism-specific biosystem; Cell adhesion molecules (CAMs), organism-specific biosystem; Cell adhesion molecules (CAMs), conserved biosystem; Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis,
Function : binding; cell adhesion molecule binding; glycolipid binding; protease binding; protein binding; sugar binding;

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