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Recombinant Mouse Tek Protein, Fc-tagged

Cat.No.: Tek-959M
Product Overview: Recombinant mouse Tek (NP_038718.2) (Met1-Lys744) was expressed with the Fc region of human IgG1 at the C-terminus.
Source: HEK293
Species: Mouse
Tag: Fc
Predicted N Terminal: Val 19
Form: Lyophilized from sterile PBS, pH 7.4, 5%~8% trehalose and mannitol.
Bio-activity: 1. Measured by its binding ability in a functional ELISA. 2. Immobilized mouse ANGPT2 at 10 μg/mL (100 μL/well) can bind mouse TEK-Fc. The EC50 of mouse TEK-Fc is 0.58-1.34 μg/mL.
Molecular Mass: The recombinant mouse Tek consists 964 amino acids and predicts a molecular mass of 107.7 kDa.
Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method.
Purity: >95 % as determined by SDS-PAGE.
Stability: Samples are stable for up to twelve months from date of receipt at -70ºC.
Storage: Store it under sterile conditions at -20ºC~-70ºC. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25 ug/ul. Centrifuge the vial at 4ºC before opening to recover the entire contents.
Gene Name: Tek endothelial-specific receptor tyrosine kinase [ Mus musculus ]
Official Symbol: Tek
Synonyms: TEK; endothelial-specific receptor tyrosine kinase; angiopoietin-1 receptor; STK1; mTIE2; p140 TEK; endothelial tyrosine kinase; tyrosine-protein kinase receptor TEK; tunica interna endothelial cell kinase; tyrosine-protein kinase receptor TIE-2; tyrosine
Gene ID: 21687
mRNA Refseq: NM_013690
Protein Refseq: NP_038718
MIM:
UniProt ID:
Pathway: Cell surface interactions at the vascular wall, organism-specific biosystem; Hemostasis, organism-specific biosystem; Rheumatoid arthritis, organism-specific biosystem; Rheumatoid arthritis, conserved biosystem; Tie2 Signaling, organism-specific biosystem
Function: ATP binding; growth factor binding; kinase activity; nucleotide binding; protein binding; protein kinase activity; protein tyrosine kinase activity; protein tyrosine kinase activity; receptor activity; transferase activity; transferase activity, transferr

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Optional requirements on this protein    +Expand
C-fusion    N-fusion   Non-tagged
His    GST   Fc   Others
<1.0 eu/μg    <0.1 eu/μg   <0.01 eu/μg   Not required
Monomer Isolation    Dimer Isolation    Not required
>80% by SDS-PAGE    >90% by SDS-PAGE   >95% by SDS-PAGE   Others

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