Active Recombinant Mouse Tgfb1 protein, His-tagged

• Cat.No.: Tgfb1-7348M
• Product Overview: Active Recombinant Mouse Tgfb1 protein(P04202)(279~390 aa), fused with N-terminal His tag, was expressed in E.coli.

Specification

• Species: Mouse
• Source: E.coli
• Tag: His
• Protein Length: 279-390 aa
• Form: Lyophilized from sterile 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Bio-activity: Transforming growth factor beta 1 or TGF-β1 is a polypeptide member of the transforming growth factor beta superfamily of cytokines. It is a secreted protein that performs many cellular functions, including the control of cell growth, cell proliferation, cell differentiation, and apoptosis. To test the effect of TGF-β1 on cell apoptosis, A549 cells were seeded into 96-well plates at a density of 5,000 cells/well with 1% serum standard DMEM including various concentrations of recombinant mouseTGF-β1. After incubated for 48h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10µL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 2 hours at 37°C. Proliferation of A549 cells after incubation with TGF-β1 for 48h observed by inverted microscope was shown. Cell viability was assessed by CCK-8 assay after incubation with recombinant mouse TGF-β1 for 48h. It was obvious that TGF-β1 significantly inhibit cell viability of A549 cells. The ED50 is 7.1μg/mL.
• Molecular Mass: 14.1kDa
• Purity: > 97% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Tgfb1 transforming growth factor, beta 1 [ Mus musculus ]
• Official Symbol: Tgfb1
• Synonyms: TGFB1; transforming growth factor, beta 1; transforming growth factor beta-1; TGF-beta 1; TGF-beta-1; regulatory protein; transforming growth factor-beta 1; Tgfb; Tgfb-1; TGFbeta1; TGF-beta1
• Gene ID: 21803
• mRNA Refseq: NM_011577
• Protein Refseq: NP_035707