Recombinant Mouse TNFa Protein, Met-tagged, Biotinylated

• Cat.No.: TNFa-731MB
• Product Overview: Biotinylated Recombinant Mouse TNFa Protein (NP_038721.1) (Leu80-Leu235) was expressed in E. coli, with an initial Met at the N-terminus.

Specification

• Source: E. coli
• Species: Mouse
• Tag: Met
• Form: Lyophilized from sterile Sterile PBS.
  Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant TNF consists of 157 amino acids and predicts a molecular mass of 17.4 kDa.
• Protein length: Leu80-Leu235
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 95 % as determined by SDS-PAGE.
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C
  Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.