Recombinant Mouse Trim50 Protein, Myc/DDK-tagged

• Cat.No.: Trim50-6658M
• Product Overview: Purified recombinant protein of mouse full-length tripartite motif-containing 50 (Trim50), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: E3 ubiquitin-protein ligase that ubiquitinates Beclin-1/BECN1 in a 'Lys-63'-dependent manner enhancing its binding to ULK1. In turn, promotes starvation-induced autophagy activation. Interacts also with p62/SQSTM1 protein and thereby induces the formation and the autophagy clearance of aggresome-associated polyubiquitinated proteins through HDAC6 interaction.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 55.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trim50 tripartite motif-containing 50 [ Mus musculus (house mouse) ]
• Official Symbol: Trim50
• Synonyms: TRIM50; tripartite motif-containing 50; E3 ubiquitin-protein ligase TRIM50; tripartite motif protein 50; tripartite motif-containing protein 50
• Gene ID: 215061
• mRNA Refseq: NM_178240
• Protein Refseq: NP_839971
• UniProt ID: Q810I2