Recombinant Mouse Trp53 Protein, His-tagged

• Cat.No.: Trp53-2140M
• Product Overview: Recombinant Mouse Trp53 Protein (Met1-Asp390) with N-His tag was expressed in E. coli.

Specification

• Description: This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined.
• Source: E. coli
• Species: Mouse
• Tag: His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 43.5 kDa
• Protein length: Met1-Asp390
• Purity: > 97%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in PBS or others.

Gene Information

• Gene Name: Trp53 transformation related protein 53 [ Mus musculus (house mouse) ]
• Official Symbol: Trp53
• Synonyms: Trp53; transformation related protein 53; bbl; bfy; bhy; p44; p53; Tp53; cellular tumor antigen p53; ME-delta123p53; mutant p53; p53 cellular tumor antigen; tumor suppressor p53; tumor supressor p53
• Gene ID: 22059
• mRNA Refseq: NM_011640
• Protein Refseq: NP_035770
• UniProt ID: P02340