Recombinant Mouse Trp53 Protein, Myc/DDK-tagged

• Cat.No.: Trp53-6671M
• Product Overview: Purified recombinant protein of mouse full-length transformation related protein 53 (Trp53), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mice deficient for this gene are developmentally normal but are susceptible to spontaneous tumors. Evidence to date shows that this gene contains one promoter, in contrast to alternative promoters of the human gene, and transcribes a few of splice variants which encode different isoforms, although the biological validity or the full-length nature of some variants has not been determined.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 43.5 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Trp53 transformation related protein 53 [ Mus musculus (house mouse) ]
• Official Symbol: Trp53
• Synonyms: TRP53; transformation related protein 53; cellular tumor antigen p53; tumor supressor p53; tumor suppressor p53; p53 cellular tumor antigen; bbl; bfy; bhy; p44; p53; Tp53
• Gene ID: 22059
• mRNA Refseq: NM_011640
• Protein Refseq: NP_035770
• UniProt ID: P02340