Recombinant Mouse Ube2k Protein, Myc/DDK-tagged

• Cat.No.: Ube2k-6791M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin-conjugating enzyme E2K (Ube2k), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro, in the presence or in the absence of BRCA1-BARD1 E3 ubiquitin-protein ligase complex, catalyzes the synthesis of 'Lys-48'-linked polyubiquitin chains. Does not transfer ubiquitin directly to but elongates monoubiquitinated substrate protein. Mediates the selective degradation of short-lived and abnormal proteins, such as the endoplasmic reticulum-associated degradation (ERAD) of misfolded lumenal proteins. Ubiquitinates huntingtin. May mediate foam cell formation by the suppression of apoptosis of lipid-bearing macrophages through ubiquitination and subsequence degradation of p53/TP53. Proposed to be involved in ubiquitination and proteolytic processing of NF-kappa-B; in vitro supports ubiquitination of NFKB1. Involved in stabilization of CASP12 during ER stress-mediated amyloid-beta neurotoxicity probably by inhibiting proteasome activity; in vitro ubiquitinates CASP12.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 22.4 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Ube2k ubiquitin-conjugating enzyme E2K [ Mus musculus (house mouse) ]
• Official Symbol: Ube2k
• Synonyms: UBE2K; ubiquitin-conjugating enzyme E2K (UBC1 homolog, yeast); ubiquitin-conjugating enzyme E2 K; HIP-2; E2(25K); ubiquitin-protein ligase; ubiquitin carrier protein; huntingtin interacting protein 2; huntingtin-interacting protein 2; ubiquitin-conjugating enzyme E2-25K; ubiquitin-conjugating enzyme E2(25K); ubiquitin-conjugating enzyme E2-25 kDa; Lig; Hip2; Hypg; E2-25k; AW492011; D5Ertd601e
• Gene ID: 53323
• mRNA Refseq: NM_016786
• Protein Refseq: NP_058066
• UniProt ID: P61087