Recombinant Full Length Human ULK1 Protein, C-Flag-tagged

• Cat.No.: ULK1-275HFL
• Product Overview: Recombinant Full Length Human ULK1 Protein, fused to Flag-tag at C-terminus, was expressed in Mammalian cells.

Specification

• Description: Serine/threonine-protein kinase involved in autophagy in response to starvation. Acts upstream of phosphatidylinositol 3-kinase PIK3C3 to regulate the formation of autophagophores, the precursors of autophagosomes. Part of regulatory feedback loops in autophagy: acts both as a downstream effector and negative regulator of mammalian target of rapamycin complex 1 (mTORC1) via interaction with RPTOR. Activated via phosphorylation by AMPK and also acts as a regulator of AMPK by mediating phosphorylation of AMPK subunits PRKAA1, PRKAB2 and PRKAG1, leading to negatively regulate AMPK activity. May phosphorylate ATG13/KIAA0652 and RPTOR; however such data need additional evidences. Plays a role early in neuronal differentiation and is required for granule cell axon formation. May also phosphorylate SESN2 and SQSTM1 to regulate autophagy.
• Source: Mammalian cells
• Species: Human
• Tag: Flag
• Form: 25 mM Tris HCl, pH 7.3, 100 mM glycine, 10% glycerol.
• Molecular Mass: 112.4 kDa
• AA Sequence: MEPGRGGTETVGKFEFSRKDLIGHG AFAVVFKGRHREKHDLEVAVKCINK KNLAKSQTLLGKEIKILKEL KHENIVALYDFQEMANSVYLVMEYC NGGDLADYLHAMRTLSEDTIRLFLQ QIAGAMRLLHSKGIIHRDLK PQNILLSNPAGRRANPNSIRVKIAD FGFARYLQSNMMAATLCGSPMYMAP EVIMSQHYDGKADLWSIGTI VYQCLTGKAPFQASSPQDLRLFYEK NKTLVPTIPRETSAPLRQLLLALLQ RNHKDRMDFDEFFHHPFLDA SPSVRKSPPVPVPSYPSSGSGSSSS SSSTSHLASPPSLGEMQQLQKTLAS PADTAGFLHSSRDSGGSKDS SCDTDDFVMVPAQFPGDLVAEAPSA KPPPDSLMCSGSSLVASAGLESHGR TPSPSPPCSSSPSPSGRAGP FSSSRCGASVPIPVPTQVQNYQRIE RNLQSPTQFQTPRSSAIRRSGSTSP LGFARASPSPPAHAEHGGVL ARKMSLGGGRPYTPSPQVGTIPERP GWSGTPSPQGAEMRGGRSPRPGSSA PEHSPRTSGLGCRLHSAPNL SDLHVVRPKLPKPPTDPLGAVFSPP QASPPQPSHGLQSCRNLRGSPKLPD FLQRNPLPPILGSPTKAVPS FDFPKTPSSQNLLALLARQGVVMTP PRNRTLPDLSEVGPFHGQPLGPGLR PGEDPKGPFGRSFSTSRLTD LLLKAAFGTQAPDPGSTESLQEKPM EIAPSAGFGGSLHPGARAGGTSSPS PVVFTVGSPPSGSTPPQGPR TRMFSAGPTGSASSSARHLVPGPCS EAPAPELPAPGHGCSFADPITANLE GAVTFEAPDLPEETLMEQEH TEILRGLRFTLLFVQHVLEIAALKG SASEAAGGPEYQLQESVVADQISLL SREWGFAEQLVLYLKVAELL SSGLQSAIDQIRAGKLCLSSTVKQV VRRLNELYKASVVSCQGLSLRLQRF FLDKQRLLDRIHSITAERLI FSHAVQMVQSAALDEMFQHREGCVP RYHKALLLLEGLQHMLSDQADIENV TKCKLCIERRLSALLTGICATRTRP LEQKLISEEDLAANDILDYKDDDDK V
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining.
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade.
• Concentration: >50 ug/mL as determined by microplate BCA method.
• Preparation: Recombinant protein was captured through anti-DDK affinity column followed by conventional chromatography steps.
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: mTOR signaling pathway, Regulation of autophagy

Gene Information

• Gene Name: ULK1 unc-51 like autophagy activating kinase 1 [ Homo sapiens (human) ]
• Official Symbol: ULK1
• Synonyms: ATG1; ATG1A; UNC51; hATG1; Unc51.1
• Gene ID: 8408
• mRNA Refseq: NM_003565.4
• Protein Refseq: NP_003556.2
• MIM: 603168
• UniProt ID: O75385