Recombinant Mouse Usp28 Protein, Myc/DDK-tagged

• Cat.No.: Usp28-6873M
• Product Overview: Purified recombinant protein of mouse full-length ubiquitin specific peptidase 28 (Usp28), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Deubiquitinase involved in DNA damage response checkpoint and MYC proto-oncogene stability. Involved in DNA damage induced apoptosis by specifically deubiquitinating proteins of the DNA damage pathway such as CLSPN. Also involved in G2 DNA damage checkpoint, by deubiquitinating CLSPN, and preventing its degradation by the anaphase promoting complex/cyclosome (APC/C). In contrast, it does not deubiquitinate PLK1. Specifically deubiquitinates MYC in the nucleoplasm, leading to prevent MYC degradation by the proteasome: acts by specifically interacting with FBXW7 (FBW7alpha) in the nucleoplasm and counteracting ubiquitination of MYC by the SCF(FBXW7) complex. Deubiquitinates ZNF304, hence preventing ZNF304 degradation by the proteasome and leading to the activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) in a subset of colorectal cancers (CRC) cells.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 119.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Usp28 ubiquitin specific peptidase 28 [ Mus musculus (house mouse) ]
• Official Symbol: Usp28
• Synonyms: USP28; ubiquitin specific peptidase 28; ubiquitin carboxyl-terminal hydrolase 28; ubiquitin thioesterase 28; deubiquitinating enzyme 28; ubiquitin thiolesterase 28; ubiquitin specific protease 28; ubiquitin-specific-processing protease 28; AU022237; mKIAA1515; 9830148O20Rik
• Gene ID: 235323
• mRNA Refseq: NM_175482
• Protein Refseq: NP_780691
• UniProt ID: Q5I043