Recombinant Mouse Vav3 Protein, Myc/DDK-tagged

• Cat.No.: Vav3-6906M
• Product Overview: Purified recombinant protein of mouse full-length vav 3 oncogene (Vav3), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: Exchange factor for GTP-binding proteins RhoA, RhoG and, to a lesser extent, Rac1. Binds physically to the nucleotide-free states of those GTPases. Plays an important role in angiogenesis. Its recruitment by phosphorylated EPHA2 is critical for EFNA1-induced RAC1 GTPase activation and vascular endothelial cell migration and assembly. May be important for integrin-mediated signaling, at least in some cell types. In osteoclasts, along with SYK tyrosine kinase, required for signaling through integrin alpha-v/beta-1 (ITAGV-ITGB1), a crucial event for osteoclast proper cytoskeleton organization and function. This signaling pathway involves RAC1, but not RHO, activation. Necessary for proper wound healing. In the course of wound healing, required for the phagocytotic cup formation preceding macrophage phagocytosis of apoptotic neutrophils. Responsible for integrin beta-2-mediated macrophage adhesion and, to a lesser extent, contributes to beta-3-mediated adhesion. Does not affect integrin beta-1-mediated adhesion.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 33.1 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Vav3 vav 3 oncogene [ Mus musculus (house mouse) ]
• Official Symbol: Vav3
• Synonyms: VAV3; vav 3 oncogene; guanine nucleotide exchange factor VAV3; VAV-3; Idd18.1; AA986410; A530094I06Rik; MGC27838
• Gene ID: 57257
• mRNA Refseq: NM_146139
• Protein Refseq: NP_666251
• UniProt ID: Q9R0C8