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Recombinant Mouse Vim protein, His-tagged

Cat.No. : Vim-251M
Product Overview : Recombinant Mouse Vim(NP_035831.2)(Ser2~Glu466) fused with His tag was expressed in E. coli.
  • Specification
  • Gene Information
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Source : E. coli
Species : Mouse
Tag : His
Form : PBS, pH7.4, containing 0.01% SKL, 1mM DTT, 5% Trehalose and Proclin300.
Molecular Mass : 52kDa as determined by SDS-PAGE reducing conditions.
Protein length : Ser2~Glu466
AA Sequence : STRSVSSSSYRRMFGGSGTSSRPSS NRSYVTTSTRTYSLGSALRPSTSRS LYSSSPGGAYVTRSSAVRLRSSVPG VRLLQDSVDFSLADAINTEFKNTRT NEKVELQELNDRFANYIDKVRFLEQ QNKILLAELEQLKGQGKSRLGDLYE EEMRELRRQVDQLTNDKARVEVERD NLAEDIMRLREKLQEEMLQREEAES TLQSFRQDVDNASLARLDLERKVES LQEEIAFLKKLHDEEIQELQAQIQE QHVQIDVDVSKPDLTAALRDVRQQY ESVAAKNLQEAEEWYKSKFADLSEA ANRNNDALRQAKQESNEYRRQVQSL TCEVDALKGTNESLERQMREMEENF ALEAANYQDTIGRLQDEIQNMKEEM ARHLREYQDLLNVKMALDIEIATYR KLLEGEESRISLPLPTFSSLNLRET NLESLPLVDTHSKRTLLIKTVETRD GQVINETSQHHDDLE
Endotoxin : <1.0EU per 1µg (determined by the LAL method)
Purity : > 97%
Applications : Positive Control; Immunogen; SDS-PAGE; WB.
If bio-activity of the protein is needed, please check active protein.
Notes : The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
Stability : The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Storage : Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
Concentration : 200µg/mL
Reconstitution : Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
Gene Name : Vim vimentin [ Mus musculus (house mouse) ]
Official Symbol : Vim
Gene ID : 22352
mRNA Refseq : NM_011701.4
Protein Refseq : NP_035831.2
UniProt ID : P20152

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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