Recombinant Mouse Wwp2 Protein, Myc/DDK-tagged

• Cat.No.: Wwp2-7015M
• Product Overview: Purified recombinant protein of Mouse WW domain containing E3 ubiquitin protein ligase 2 (Wwp2), with C-terminal MYC/DDK tag, expressed in HEK293T cells.

Specification

• Description: E3 ubiquitin-protein ligase which accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates. Polyubiquitinates POU5F1 by 'Lys-63'-linked conjugation and promotes it to proteasomal degradation; regulates POU5F1 protein level during differentiation of embryonal carcinoma cells (ECCs) but not in undifferentiated ECCs and embryonic stem cells (ESCs). Ubiquitinates EGR2 and promotes it to proteasomal degradation; in T-cells the ubiquitination inhibits activation-induced cell death. Ubiquitinates SLC11A2; the ubiquitination is enhanced by presence of NDFIP1 and NDFIP2. Ubiquitinates RPB1 and promotes it to proteasomal degradation.
• Source: HEK293T
• Species: Mouse
• Tag: Myc/DDK
• Molecular Mass: 98.8 kDa
• Purity: > 80% as determined by SDS-PAGE and Coomassie blue staining
• Stability: Stable for 12 months from the date of receipt of the product under proper storage and handling conditions. Avoid repeated freeze-thaw cycles.
• Storage: Store at -80 centigrade after receiving vials.
• Concentration: >50 μg/mL as determined by microplate BCA method
• Storage Buffer: 25 mM Tris.HCl, pH 7.3, 100 mM glycine, 10% glycerol.

Gene Information

• Gene Name: Wwp2 WW domain containing E3 ubiquitin protein ligase 2 [ Mus musculus (house mouse) ]
• Official Symbol: Wwp2
• Synonyms: WWP2; WW domain containing E3 ubiquitin protein ligase 2; NEDD4-like E3 ubiquitin-protein ligase WWP2; WW domain-containing protein 2; WW domain-containing protein 4; AIP2; AA690238; AW554328; 1300010O06Rik
• Gene ID: 66894
• mRNA Refseq: NM_025830
• Protein Refseq: NP_080106
• UniProt ID: Q9DBH0