Recombinant Porcine Interferon-gamma

• Cat.No.: IFNG-21P
• Product Overview: Recombinant Porcine Interferon-gamma produced inE.Coliis a single, non-glycosylated, polypeptide chain containing 146 amino acids and having a molecular mass of 17140 Dalton. The IFN-gamma is purified by proprietary chromatographic techniques.

Specification

• Species: Pig
• Source: E.coli
• Tag: Non
• Description: IFN-gamma produced by lymphocytes activated by specific antigens or mitogens. IFN-gamma, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons.
• Amino Acid Sequence: The sequence of the first five N-terminal amino acids was determined and was found to be Ser-Tyr-Cys-Gln-Ala.
• Physical Appearance: Sterile Filtered White lyophilized (freeze-dried) powder.
• Purity: Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE.
• Formulation: The protein was lyophilized with no additives.
• Solubility: It is recommended to reconstitute the lyophilized Interferon-gamma in sterile 18MΩ-cm H₂O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
• Biological Activity: The ED₅₀as determined by theamount of interferon that inhibited 50% of the cytopathic effect of vesicular stomatitis virus in MDBK cells was < 0.3 ng/ml.
• Protein Content: Protein quantitation was carried out by two independent methods: 1.UV spectroscopy at 280 nm using the absorbency value of 0.556 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a calibrated solution of IFN as a Reference Standard.
• Storage: Lyophilized Interferon-g although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IFN gamma should be stored at 4℃ between 2-7 days and for future use below -18℃. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Gene Information

• Gene Name: IFNG interferon-gamma [ Sus scrofa ]
• Synonyms: IFNG; Interferon-gamma; IFN-gamma Interferon gamma; Interferon, gamma; IFG; IFI; IFN-gamma; Immune interferon; Type II interferon; T cell interferon; MAF
• Gene ID: 396991
• mRNA Refseq: NM_213948
• Protein Refseq: NP_999113
• UniProt ID: P17803
• Chromosome Location: 5p1.2-q1.1
• Pathway: Allograft rejection; Cytokine-cytokine receptor interaction; Graft-versus-host disease; Jak-STAT signaling pathway; Natural killer cell mediated cytotoxicity; Proteasome; Regulation of autophagy; Systemic lupus erythematosus; T cell receptor signaling pathway; TGF-beta signaling pathway; Type I diabetes mellitus