Cat. No. : |
IL4-84P |
Description : |
IL4 is a ligand for interleukin 4 receptor. The interleukin 4 receptor also binds to IL13, STAT6, a signal transducer and activator of transcription, has been shown to play a central role in mediating the immune regulatory signal of this cytokine. This gene, IL3, IL5, IL13, and CSF2 form a cytokine gene cluster on chromosome 5q, with this gene particularly close to IL13. IL4, IL13 and IL5 are found to be regulated coordinately by several long-range regulatory elements in an over 120 kilobase range on the chromosome. |
Source : |
Escherichia Coli. |
Amino Acid Sequence : |
The sequence of the first five N-terminal amino acids was determined and was found to be Met-His-Ly-Lys-Asp. |
Physical Appearance : |
Sterile Filtered White lyophilized (freeze-dried) powder. |
Purity : |
Greater than 95.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
Formulation : |
Lyophilized from a concentrated (1 mg/ml) solution in water containing no additives. |
Solubility : |
It is recommended to reconstitute the lyophilized Interleikin-4 in sterile 18 MΩ-cm H2O not less than 100 µg/ml, which can then be further diluted to other aqueous solutions. |
Specific Activity : |
The ED50 range= 1 to 4ng/ml. The biological activity is determined by measuring the dose dependent proliferation of human TF-1 cells. A concentration range of 0.1 to 10.0ng/ml is effective for most in vitro applications. |
Protein Content : |
Protein quantitation was carried out by two independent methods: 1. UV spectroscopy at 280 nm using the absorbency value of 0.218 as the extinction coefficient for a 0.1% (1mg/ml) solution. This value is calculated by the PC GENE computer analysis program of protein sequences (IntelliGenetics). 2. Analysis by RP-HPLC, using a standard solution of IL-4 as a Reference Standard. |
Stability : |
Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18℃. Upon reconstitution IL4 should be stored at 4℃ between 2-7 days and for future use below -18℃. Please prevent freeze-thaw cycles. |