Recombinant Protein G-Firefly Luciferase Fusion Protein, His-tagged

• Cat.No.: Luciferase-11F
• Product Overview: Recombinant Protein G-Firefly Luciferase Fusion Protein, fused with His tag, was expressed in E. coli.

Specification

• Species: Firefly
• Source: E. coli
• Tag: His
• Description: Antibody Binding Firefly Luciferase is a Protein G-Firefly Luciferase (protein G-Fluc) fusion protein. It consists of protein G and firefly luciferase (Fluc), while the two proteins connect via a short peptide. Protein G tightly binds to IgG molecules from rabbit, human, mouse, goat, sheep, cow, and rat. In addition, protein G conjugates are especially valuable for detecting antibodies that bind poorly to protein A, such as human IgG3, mouse IgG1, and immunoglobulins from rat, sheep, and horse. Firefly luciferase (Fluc) is a light-emitting enzyme to estimate gene expression. It catalyzes the oxidation of firefly luciferin, allowing its detection by a luminometer. As an antibody binding luciferase, protein G-Fluc can avoid harmful labeling of the detecting antibody. Because staining protocols vary with application, the appropriate dilution of Antibody Binding Firefly Luciferase should be determined empirically.
• Form: 50 mM sodium phosphate,100 mM NaCl, 1 mM DTT, pH7.2, 50% glycerol
• Molecular Mass: 85.3 kDa
• Purity: ≥ 90% by SDS-PAGE
• Storage: Store at -20°C.
• Concentration: 0.5 ~ 1.0 mg/ml
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.