| Description : |
LUC, also known as luciferase. LUC catalyzes the oxidative decarboxylation of coelenterazine in the presence of dissolvedoxygen to yield oxyluciferin, CO2, and blue light. In vivo, the excited state luciferin-luciferase complex undergoes the process of noradiative energy transfer to an accessory protein, green fluorescent protein, which results in green bioluminescence. In vitro, it emits blue light in the absence of any green fluorescent protein. |
| Source : |
E. coli |
| Species : |
Renilla reniformis |
| Tag : |
N-His |
| Form : |
Liquid |
| Bio-activity : |
Specific activity is >1×10^9 lightunit/mg. One luciferase enzymeunit will produce one Relative Light Unit (RLU) at pH 7.5 at 25 centigrade. |
| Molecular Mass : |
38.5 kDa (335aa) Confirmed by MALDI-TOF |
| Protein Length : |
1-311 |
| AA Sequence : |
MTSKVYDPEQRKRMITGPQWWARCK QMNVLDSFINYYDSEKHAENAVIFL HGNAASSYLWRHVVPHIEPVARCII PDLIGMGKSGKSGNGSYRLLDHYKY LTAWFELLNLPKKIIFVGHDWGACL AFHYSYEHQDKIKAIVHAESVVDVI ESWDEWPDIEEDIALIKSEEGEKMV LENNFFVETMLPSKIMRKLEPEEFA AYLEPFKEKGEVRRPTLSWPREIPL VKGGKPDVVQIVRNYNAYLRASDDL PKMFIESDPGFFSNAIVEGAKKFPN TEFVKVKGLHFSQEDAPDEMGKYIK SFVERVLKNEQ |
| Endotoxin : |
< 1 EU/μg of protein (determined by LAL method) |
| Purity : |
> 95% by SDS-PAGE |
| Applications : |
SDS-PAGE, Enzyme Activity |
| Storage : |
Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles. |
| Concentration : |
0.5 mg/mL (determined by Bradford assay) |
| Storage Buffer : |
20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol |
| Official Symbol : |
Luciferase |
| Synonyms : |
Luciferase; Coelenterazine h 2-monooxygenase; EC:1.13.12.5; Renilla-luciferin 2-monooxygenase; Renilla-type luciferase |
| UniProt ID : |
P27652 |
