Active Recombinant Renilla reniformis Luciferase (1-311), N-His-tagged

• Cat.No.: Luciferase-10R
• Product Overview: Recombinant renilla reniformis LUC (1-311), fused to His-tag at N-terminus, was expressed in E. coli and purified by using conventional chromatography techniques.

Specification

• Description: LUC, also known as luciferase. LUC catalyzes the oxidative decarboxylation of coelenterazine in the presence of dissolvedoxygen to yield oxyluciferin, CO2, and blue light. In vivo, the excited state luciferin-luciferase complex undergoes the process of noradiative energy transfer to an accessory protein, green fluorescent protein, which results in green bioluminescence. In vitro, it emits blue light in the absence of any green fluorescent protein.
• Source: E. coli
• Species: Renilla reniformis
• Tag: His
• Form: Liquid
• Bio-activity: Specific activity is >1×10^9 lightunit/mg. One luciferase enzymeunit will produce one Relative Light Unit (RLU) at pH 7.5 at 25 centigrade.
• Molecular Mass: 38.5 kDa (335aa) Confirmed by MALDI-TOF
• Protein length: 1-311
• AA Sequence: MTSKVYDPEQRKRMITGPQWWARCK QMNVLDSFINYYDSEKHAENAVIFL HGNAASSYLWRHVVPHIEPVARCII PDLIGMGKSGKSGNGSYRLLDHYKY LTAWFELLNLPKKIIFVGHDWGACL AFHYSYEHQDKIKAIVHAESVVDVI ESWDEWPDIEEDIALIKSEEGEKMV LENNFFVETMLPSKIMRKLEPEEFA AYLEPFKEKGEVRRPTLSWPREIPL VKGGKPDVVQIVRNYNAYLRASDDL PKMFIESDPGFFSNAIVEGAKKFPN TEFVKVKGLHFSQEDAPDEMGKYIK SFVERVLKNEQ
• Endotoxin: < 1 EU/μg of protein (determined by LAL method)
• Purity: > 95% by SDS-PAGE
• Applications: SDS-PAGE, Enzyme Activity
• Storage: Can be stored at +2 to +8 centigrade for 1 week. For long term storage, aliquot and store at -20 to -80 centigrade. Avoid repeated freezing and thawing cycles.
• Concentration: 0.5 mg/mL (determined by Bradford assay)
• Storage Buffer: 20mM Tris-HCl buffer (pH 8.0) containing 1mM DTT, 10% glycerol
• Official Symbol: Luciferase
• Synonyms: Luciferase; Coelenterazine h 2-monooxygenase; EC:1.13.12.5; Renilla-luciferin 2-monooxygenase; Renilla-type luciferase
• UniProt ID: P27652

Gene Information

• Official Symbol: Luciferase
• Synonyms: Luciferase; Coelenterazine h 2-monooxygenase; EC:1.13.12.5; Renilla-luciferin 2-monooxygenase; Renilla-type luciferase
• UniProt ID: P27652

Reviews

05/18/2022

Worked well, happy customer.

08/30/2021

Great in lab experiments.

09/04/2020

Satisfied with the purchase.

Q&As

How is luciferase utilized in bioluminescence imaging?

It allows for real-time tracking of cellular and molecular processes in living organisms.

What is the primary function of luciferase in organisms that produce it?

Luciferase enables bioluminescence, helping organisms attract mates, prey, or deter predators.

How does luciferase produce light in a biochemical reaction?

It catalyzes the oxidation of luciferin, releasing light.

In which organisms is luciferase commonly found?

Luciferase is found in fireflies, certain jellyfish, and some fungi and bacteria.

Are there different types of luciferase, and how do they vary?

Yes, there are different types, varying in their substrate specificity and the color of emitted light.

What is the role of ATP in the luciferase reaction?

ATP provides energy for the luciferase reaction, converting luciferin to oxyluciferin.

What advantages does the use of luciferase offer in scientific research?

Luciferase provides a non-invasive and highly sensitive method for studying biological phenomena.