Recombinant Pseudomonas aeruginosa lox Protein (M1-I678), Flag/His-tagged

• Cat.No.: lox-1565P
• Product Overview: Recombinant Pseudomonas aeruginosa Flag-6His-TEV-G-lipoxygenase(M1-I678 end) protein with a Flag/His tag was expressed in E. coli

Specification

• Species: Pseudomonas aeruginosa
• Source: E.coli
• Tag: Flag&His
• Protein Length: M1-I678
• Description: In presence of oxygen, converts linoleate into (9S)-hydroperoxy-10,12-octadecenoate (9HPOD), which spontaneously decomposes to the corresponding 9-hydroxy-10,12-octadecenoate (9HOD), and into 13-hydroperoxy-9,11-octadecenoate (13HPOD) which spontaneously decomposes to the corresponding 13-hydroxy-9,11-octadecenoate (13HOD). Also active on linolenate. To a lesser extent, is also able to convert oleate into (10S)-hydroperoxy-8E-octadecenoate, which spontaneously decomposes to the corresponding 10-hydroxy-8E-octadecenoate. Is almost not active on arachidonate.
• Form: Liquid
• Endotoxin: < 0.01 EU/μg of the protein
• Purity: 90%
• Stability: Samples are stable for up to twelve months from date of receipt at -20 to -80 centigrade
• Storage: Store it under sterile conditions at -20 to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Storage Buffer: Supplied as sterile 50mM Tris-HCl (pH 7.5), 200mM NaCl, 20% glycerol
• Shipping: It is shipped out with blue ice.

Gene Information

• Official Symbol: lox
• Synonyms: Linoleate 9/13-lipoxygenase; lox; EC:1.13.11.12; Oleate 10S-lipoxygenase; EC:1.13.11.77; EC:1.13.11.58
• UniProt ID: Q8RNT4