Recombinant Rat Abcg8 protein, His & T7-tagged

• Cat.No.: Abcg8-8152R
• Product Overview: Recombinant Rat Abcg8 aa. (Met1~Gln271 (Accession # P58428)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Description: ATP-binding cassette (ABC) transporter that regulates sterol absorption and excretion; mutation causes sitosterolemia.
• Source: E. coli
• Species: Rat
• Tag: His & T7
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.1kDa.
• Protein length: Met1~Gln271 (Accession # P58428)
• Endotoxin: <1.0EU per 1ug (determined by the LAL method)
• Purity: >95%
• Characteristic: The isoelectric point is 9.5.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. (Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.) The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage Buffer: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative.
• Reconstitution: Reconstitute in sterile ddH2O.

Gene Information

• Gene Name: Abcg8 ATP binding cassette subfamily G member 8 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Abcg8
• Synonyms: Abcg8; ATP binding cassette subfamily G member 8; ATP-binding cassette sub-family G member 8; ATP-binding cassette, sub-family G (WHITE), member 8; ATP-binding cassette, subfamily G (WHITE), member 8; sterolin 2
• Gene ID: 155192
• mRNA Refseq: NM_130414.2
• Protein Refseq: NP_569098.2
• UniProt ID: P58428

Reviews

08/01/2019

Good work in immunohistochemistry

04/21/2018

The purification rsults demonstrtaed clearly! Great.

Q&As

What may happen if the allele in ABCG8, NPC1L1 rs217434 mutated?

To a lesser extent, NPC1L1 rs217434 variants increase the risk of early-onset gallstone formation.

what biological variation may influence the association between ABCG8 transporters and hypercholesterolemia?

Single nucleotide polymorphisms (SNPs) in ABCG8 transporter have been associated with hypercholesterolemia.

What will happen if disrupt both ABCG5 and ABCG8 in mice?

lt will cause extremely low biliary cholesterol concentrations while biliary phospholipids and bile acid concentrations are not altered.

What is the relationship between ABCG8 and leptin in mice?

Mice lacking leptin, exhibit decreased abundance of ABCG8 proteins in the liver.

What will the overexpression of ABCG8 genes cause?

It will potentially induce atherosclerosis because of reduced circulating and hepatic cholesterol.

What results can you found through western blot experiment results?

both anti-ABCG5 and anti-ABCG8 antibodies bound to ~75 kDa proteins in human liver crude membranes.

What is the difference between deficient in either Abcg5 or Abcg8 and double missing?

One study constructed mouse models deficient in either Abcg5 or Abcg8 that show different biliary physiology than that of the Abcg5/Abcg8 double knockout mice. This suggests these proteins may have independent function(s) in addition to their function as heterodimers.

What is the distribution pattern of ABCG8 in small intestine ?

 ABCG8 appear to line the brush border.

What changes can affect the expression of ABCG8?

Disruptions of bile acid metabolism alter hepatic ABCG5/G8 expression.

What is the relationship between ABCG5 and ABCG8?

ABCG5 and ABCG8 are co-regulated at the transcriptional level, sharing a common bidirectional promoter of 374 base pairs that separates their initiation codons, cause sitosterolemia as well.