Active Recombinant Rat Acvr2b protein, His-tagged, Low Endotoxin

• Cat.No.: ACVR2B-151R
• Product Overview: Recombinant Rat Acvr2b protein(P38445)(19-137 aa), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 19-137 aa
• Form: Lyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
• Bio-activity: Immobilized Human Activin A, No Tag at 1μg/ml (100μl/well) on the plate. Dose response curve for Rat Activin RIIB, His Tag with the EC50 of 12.7ng/ml determined by ELISA.
• Molecular Mass: The protein has a predicted MW of 15.29 kDa. Due to glycosylation, the protein migrates to 30-50 kDa based on Bis-Tris PAGE result.
• Endotoxin: Less than 0.01 EU per μg by the LAL method.
• Purity: > 95% as determined by Bis-Tris PAGE; > 95% as determined by HPLC
• Storage: -20 to -80°C for 12 months as supplied from date of receipt. -80°C for 3 months after reconstitution. Recommend to aliquot the protein into smaller quantities for optimal storage. Please minimize freeze-thaw cycles.
• Reconstitution: Dissolve the lyophilized protein in distilled water.

Gene Information

• Gene Name: Acvr2b activin A receptor, type IIB [ Rattus norvegicus ]
• Official Symbol: Acvr2b
• Synonyms: ACVR2B; activin A receptor, type IIB; activin receptor type-2B; ACTR-IIB; activin receptor IIB; activin receptor type IIB; ActRIIB type II activin receptor B; Activine receptor 2b (transmembrane serine kinase)
• Gene ID: 25366
• mRNA Refseq: NM_031554
• Protein Refseq: NP_113742