Recombinant Rat Ccl24 protein

• Cat.No.: Ccl24-638R
• Product Overview: Recombinant Rat Ccl24 protein was expressed in Escherichia coli.

Specification

• Description: CCL24, also named MPIF-2, Eotaxin-2 and Ckβ6, is a novel CC chemokine recently identified. It is a secreted protein, encoded by CCL24 gene, and produced by activated monocytes and T lymphocytes. CCL24 signals through the CCR3 receptor and has functions of chemotactic activity for resting T-lymphocytes and eosinophils, but none for monocytes and activated lymphocytes. The plasma levels of CCL24 and the aspirin-exacerbated respiratory disease (such as asthma) morbidity rate have positive correlation.
• Source: E.coli
• Species: Rat
• Form: Lyophilized from a 0.2μm filtered concentrated solution in 2 × PBS, pH 7.4.
• Bio-activity: Fully biologically active when compared to standard. The biologically active determined by a chemotaxis bioassay using human peripheral blood eosinophils is in a concentration of 50-250 ng/ml.
• Molecular Mass: Approximately 10.5 kDa, a single non-glycosylated polypeptide chain consisting of an N-terminal Methionine and the mature rat Eotaxin-2.
• Protein length: 98
• AA Sequence: MPTGSVTIPSSCCVTFISKKIPVNR VISYQLANGSICPKAGVIFITKKGH KICTDPKLPWVQKHIKNLDAKRNQP SEGAKALGPKFVIQKLRGNSTKV
• Endotoxin: Less than 1 EU/µg of rRtEotaxin-2/CCL24 as determined by LAL method.
• Purity: >96% by SDS-PAGE and HPLC analysis.
• Storage: Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
• Reconstitution: We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
• Tag: Non

Gene Information

• Gene Name: Ccl24
• Official Symbol: Ccl24
• Synonyms: CCL24; chemokine (C-C motif) ligand 24; C-C motif chemokine 24; eotaxin-2; eotaxin-2-like protein; eosinophil chemotactic protein 2
• Gene ID: 288593
• mRNA Refseq: NM_001013045
• Protein Refseq: NP_001013063
• UniProt ID: Q5PPP2