Recombinant Rat Chemokine (C-C Motif) Ligand 5

Cat.No. : Ccl5-104R
Product Overview : Recombinant Rat RANTESproduced inE.coliis a 7.9 kDa protein containing 69 amino acid residues.
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Species : Rat
Source : E.coli
Tag : Non
Description : RANTES is a protein which has been shown to be a chemoattractant for peripheral blood monocytes. It appears to selectively attract T cells of the CD4+/CD45RO+ phenotype in vitro.
Purity : > 98% (SDS-PAGE; HPLC).
Endotoxin level : < 0.1 ng/g of RANTES.
Stabilizer : None.
Formulation : Lyophilized.
Biologically Activity : Rat RANTES produces chemotaxis over a wide concentration range in an assay using rat peritoneal macrophages. Significant chemotaxis was achieved using a concentration of 10-50 ng/ml.
Reconstitution : The lyophilized RANTES is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water to a concentration of 100 ng/µl. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.
Stability : The lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted RANTES should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
Publications :
Control of transplant tolerance and intragraft regulatory T cell localization by myeloid-derived suppressor cells and CCL5 (2012)
Gene Name Ccl5 chemokine (C-C motif) ligand 5 [ Rattus norvegicus ]
Synonyms Ccl5; chemokine (C-C motif) ligand 5; Scya5; Rantes; small inducible cytokine A5; RANTES; small inducible cytokine A5 (RANTES)
Gene ID 81780
mRNA Refseq NM_031116
Protein Refseq NP_112378
UniProt ID P50231
Chromosome Location q26
Pathway Chemokine signaling pathway; Cytokine-cytokine receptor interaction; Prion diseases; Toll-like receptor signaling pathway
Function chemoattractant activity; chemokine activity

Control of transplant tolerance and intragraft regulatory T cell localization by myeloid-derived suppressor cells and CCL5.

Journal: Journal of immunology (Baltimore, Md. : 1950)    PubMed ID: 22450806    Data: 2012/6/11

Authors: Nahzli Dilek, Nicolas Poirier, Bernard Vanhove

Article Snippet:ALZET osmotic mini-pumps (model 2001) were purchased from Charles River (Wilmington, MA).ALZET osmotic mini-pumps (model 2001) were purchased from Charles River (Wilmington, MA).. Filling osmotic pumps with recombinant rat CCL5 (Creative BioMart) was done according to the instructions of the manufacturer.. Pumps were surgically implanted i.p. under isoflurane anesthesia and sterile conditions.Pumps were surgically implanted i.p. under isoflurane anesthesia and sterile conditions.

Not For Human Consumption!

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