Recombinant Rat Chemokine (C-C Motif) Ligand 5

• Cat.No.: Ccl5-104R
• Product Overview: Recombinant Rat RANTESproduced inE.coliis a 7.9 kDa protein containing 69 amino acid residues.

Specification

• Species: Rat
• Source: E.coli
• Tag: Non
• Description: RANTES is a protein which has been shown to be a chemoattractant for peripheral blood monocytes. It appears to selectively attract T cells of the CD4+/CD45RO+ phenotype in vitro.
• Purity: > 98% (SDS-PAGE; HPLC).
• Endotoxin level: < 0.1 ng/g of RANTES.
• Stabilizer: None.
• Formulation: Lyophilized.
• Biologically Activity: Rat RANTES produces chemotaxis over a wide concentration range in an assay using rat peritoneal macrophages. Significant chemotaxis was achieved using a concentration of 10-50 ng/ml.
• Reconstitution: The lyophilized RANTES is soluble in water and most aqueous buffers. The lyophilized powder should be reconstituted in water to a concentration of 100 ng/µl. It is recommended that further dilutions be made into buffer containing carrier protein or medium containing serum.
• Stability: The lyophilized powder, though stable at room temperature, is best stored desiccated below 0°C. Reconstituted RANTES should be stored in working aliquots at -20°C. Avoid repeated freeze-thaw cycles.
• Publications:
  Control of transplant tolerance and intragraft regulatory T cell localization by myeloid-derived suppressor cells and CCL5 (2012)

Gene Information

• Gene Name: Ccl5 chemokine (C-C motif) ligand 5 [ Rattus norvegicus ]
• Synonyms: Ccl5; chemokine (C-C motif) ligand 5; Scya5; Rantes; small inducible cytokine A5; RANTES; small inducible cytokine A5 (RANTES)
• Gene ID: 81780
• mRNA Refseq: NM_031116
• Protein Refseq: NP_112378
• UniProt ID: P50231
• Chromosome Location: q26
• Pathway: Chemokine signaling pathway; Cytokine-cytokine receptor interaction; Prion diseases; Toll-like receptor signaling pathway
• Function: chemoattractant activity; chemokine activity