Recombinant Rat Cyp7a1 protein, His & T7-tagged

• Cat.No.: Cyp7a1-1441R
• Product Overview: Recombinant Rat Cyp7a1 aa. (Phe109~Lys383 (Accession # P18125)) fused with N-terminal His & T7 tag was produced in E. coli cells.

Specification

• Species: Rat
• Source: E.coli
• Tag: His&T7
• Protein Length: Phe109~Lys383
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 34.5kDa.
• Endotoxin: <1.0EU per 1µg (determined by the LAL method).
• Purity: >95%
• Characteristic: The isoelectric point is 6.9.
• Applications: SDS-PAGE; WB; ELISA; IP.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8°C for one month. Aliquot and store at -80°C for 12 months.
• Storage buffer: Supplied as lyophilized form in PBS, pH7.4, containing 5% trehalose, 0.01% sarcosyl.
• Reconstitution: Reconstitute in sterile PBS, pH7.2-pH7.4.

Gene Information

• Gene Name: Cyp7a1 cytochrome P450 family 7 subfamily A member 1 [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Cyp7a1
• Synonyms: CHAP; CYP7; CYP7S1; CYPVII; cholesterol 7-alpha-hydroxylase; cytochrome P450 (cholesterol hydroxylase 7 alpha); cytochrome P450, 7a1; cytochrome P450, family 7, subfamily a, polypeptide 1; cholesterol 7-alpha-monooxygenase
• Gene ID: 25428
• mRNA Refseq: NM_012942.2
• Protein Refseq: NP_037074.1
• UniProt ID: P18125