Active Recombinant Rat Efna5 protein, Fc-tagged

• Cat.No.: Efna5-1574R
• Product Overview: Recombinant Rat Efna5(Met 1-Glu 202) fused with Fc region of human IgG1 tag at C-terminal was expressed in HEK293.

Specification

• Source: HEK293
• Species: Rat
• Tag: Fc
• Predicted N Terminal: Phe 17
• Form: Lyophilized from sterile PBS, pH 7.41. Normally 5 % - 8 % trehalose and mannitol are added as protectants before lyophilization. Specific concentrations are included in the hardcopy of COA.
• Bio-activity: Measured by its binding ability in a functional ELISA.Immobilized recombinant Rat EPHA4 Protein (His Tag) at 10 µg/mL (100 μl/well) can bind Recombinant rat EFNA5-Fc with a linear range of 7.8-12.5 ng/ml.
• Molecular Mass: The recombinant rat EFNA5/Fc is a disulfide-linked homodimer. The reduced monomer comprises 423 amino acids and predicts a molecular mass of 48 kDa. The apparent molecular mass of the rat EFNA5/Fc monomer is approximately 55 kDa in SDS-PAGE under reducing conditions due to glycosylation.
• Protein length: Met 1-Glu 202
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Stability: Samples are stable for up to twelve months from date of receipt at -70 centigrade
• Storage: Store it under sterile conditions at -20 centigrade to -80 centigrade. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: A hardcopy of COA with reconstitution instruction is sent along with the products. Please refer to it for detailed information.
• Shipping: In general, recombinant proteins are provided as lyophilized powder which are shipped at ambient temperature.Bulk packages of recombinant proteins are provided as frozen liquid. They are shipped out with blue ice unless customers require otherwise.

Gene Information

• Gene Name: Efna5 ephrin A5 [ Rattus norvegicus ]
• Official Symbol: Efna5
• Synonyms: EFNA5; ephrin A5; ephrin-A5; AL-1; LERK-7; eph-related receptor tyrosine kinase ligand 7; Lerk7
• Gene ID: 116683
• mRNA Refseq: NM_053903
• Protein Refseq: NP_446355
• UniProt ID: P97605
• Chromosome Location: 9q37
• Pathway: Axon guidance, organism-specific biosystem; Axon guidance, conserved biosystem
• Function: chemorepellent activity; chemorepellent activity; ephrin receptor binding; ephrin receptor binding; ephrin receptor binding