| Description : |
Fibroblast growth factor 10 is a protein which belongs to the fibroblast growth factor (FGF) family and FGF family members are involved in a variety of biological processes such as embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. Like most other FGF family members, FGF-10 also has a heparin-binding domain and it plays an important role in the regulation of embryonic development, cell proliferation and cell differentiation. In addition, FGF-10 may play a role in wound healing and is required for normal branching morphogenesis. Recombinant rat FGF-10 contains a 179 amino acids and it shares 94 % and 100 % a.a. sequence identity with human and murine FGF-10. |
| Source : |
E.coli |
| Species : |
Rat |
| Form : |
Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris, 500 mM NaCl, pH 7.4, 5 % trehalose. |
| Bio-activity : |
Fully biologically active when compared to standard. The ED50 as determined by a cell proliferation assay using monkey 4MBr-5 cells is less than 120 ng/ml, corresponding to a specific activity of > 8.3 × 10³ IU/mg. |
| Molecular Mass : |
Approximately 20.0 kDa, a single non-glycosylated polypeptide chain containing 179 amino acids. |
| Protein length : |
179 |
| AA Sequence : |
QALGQDMVSPEATNSSSSSSSSSSS SSFSSPSSAGRHVRSYNHLQGDVRW RKLFSFTKYFLKIEKNGKVSGTKKE NCPYSILEITSVEIGVVAVKAINSN YYLAMNKKGKLYGSKEFNNDCKLKE RIEENGYNTYASFNWQHNGRQMYVA LNGKGAPRRGQKTRRKNTSAHFLPM VVHS |
| Endotoxin : |
Less than 1 EU/µg of rRtKGF-2/FGF-10 as determined by LAL method. |
| Purity : |
>97% by SDS-PAGE and HPLC analysis. |
| Storage : |
Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution. |
| Reconstitution : |
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions. |
