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Recombinant Rat Fgf9 protein

Cat.No. : Fgf9-112R
Product Overview : Recombinant Rat Fgf9 protein was expressed in Escherichia coli.
  • Specification
  • Gene Information
  • Related Products
Description : Fibroblast growth factor-9 (FGF-9) is a member of the fibroblast growth factor (FGF) family. All FGF family members are heparin binding growth factors with a core 120 amino acid (a.a.) FGF domain that allows for a common tertiary structure. FGF-9 plays an important role in the regulation of embryonic development, cell proliferation, cell differentiation and cell migration. This protein was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. FGF-9 is a monomer and interacts with FGFR1, FGFR2, FGFR3 and FGFR4. The rat FGF-9 shares 98 % a.a. sequence identity with human FGF-9.
Source : E.coli
Species : Rat
Form : Lyophilized from a 0.2μm filtered concentrated solution in 20 mM Tris, 400 mM NaCl, pH 8.0.
Bio-activity : Fully biologically active when compared to standard. The ED50 as determined by thymidine uptake assay using FGF-receptors transfected BaF3 cells is less than 0.5 ng/ml, corresponding to a specific activity of > 2.0 × 10⁶ IU/mg.
Molecular Mass : Approximately 23.1 kDa, a single non-glycosylated polypeptide chain containing 205 amino acids.
Protein length : 205
AA Sequence : LGEVGSYFGVQDAVPFGNVPVLPVD SPVLLSDHLGQSEAGGLPRGPAVTD LDHLKGILRRRQLYCRTGFHLEIFP NGTIQGTRKDHSRFGILEFISIAVG LVSIRGVDSGLYLGMNEKGELYGSE KLTQECVFREQFEENWYNTYSSNLY KHVDTGRRYYVALNKDGTPREGTRT KRHQKFTHFLPRPVDPDKVPELYKD ILSQS
Endotoxin : Less than 1 EU/µg of rRtFGF-9-1 as determined by LAL method.
Purity : >95% by SDS-PAGE and HPLC analysis.
Storage : Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 12 months from date of receipt, -20 to -70 centigrade as supplied. 1 month, 2 to 8 centigrade under sterile conditions after reconstitution. 3 months, -20 to -70 centigrade under sterile conditions after reconstitution.
Reconstitution : We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1 % BSA to a concentration of 0.1-1.0 mg/mL. Stock solutions should be apportioned into working aliquots and stored at ≤-20 centigrade. Further dilutions should be made in appropriate buffered solutions.
Gene Name : Fgf9
Official Symbol : Fgf9
Synonyms : GAF, Glia-activating factor, HBGF-9
Gene ID : 25444
mRNA Refseq : NM_012952
Protein Refseq : NP_037084
UniProt ID : P36364

For Research Use Only. Not intended for any clinical use. No products from Creative BioMart may be resold, modified for resale or used to manufacture commercial products without prior written approval from Creative BioMart.

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