Recombinant Rat Il13ra1 protein(Met1-Thr342), hFc-tagged

• Cat.No.: Il13ra1-7450R
• Product Overview: Recombinant Rat IL13RA1 (Q561K3) (Met1-Thr342) was expressed in HEK293, fused with the Fc region of Human IgG1 at the C-terminus.

Specification

• Source: HEK293
• Species: Rat
• Tag: Fc
• Protein length: Met1-Thr342
• Form: Lyophilized from sterile PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween80 are added as protectants before lyophilization.
• Molecular Mass: The recombinant rat IL13RA1/Fc is a disulfide-linked homodimer. The reduced monomer comprises 557 amino acids and has a predicted molecular mass of 62.9 kDa. The apparent molecular mass of the protein is approximately 91 kDa in SDS-PAGE under reducing conditions.
• Endotoxin: < 1.0 EU per μg of the protein as determined by the LAL method
• Purity: > 90 % as determined by SDS-PAGE
• Storage: Samples are stable for up to twelve months from date of receipt at -20°C to -80°C. Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.2 ug/ul. Centrifuge the vial at 4°C before opening to recover the entire contents.

Gene Information

• Gene Name: Il13ra1 interleukin 13 receptor, alpha 1 [ Rattus norvegicus ]
• Official Symbol: Il13ra1
• Synonyms: IL13RA1; interleukin 13 receptor, alpha 1; interleukin-13 receptor subunit alpha-1; MGC105309
• Gene ID: 252963
• mRNA Refseq: NM_145789
• Protein Refseq: NP_665732

Reviews

01/10/2022

High solubility.

01/17/2019

Potent in anti-allergic assays.

04/26/2018

Suitable for ELISA.

Q&As

What techniques determine IL13RA1 expression levels in different cell types?

Techniques quantify IL13RA1 expression across diverse cell types.

What experimental strategies discern IL13RA1 isoforms and their functions?

Strategies distinguish IL13RA1 isoforms and their roles.

Could you elaborate on the use of RNA-seq in profiling IL13RA1-regulated gene expression?

RNA-seq profiles IL13RA1's influence on gene expression.

What are the advantages of using CRISPR/Cas9 to knockout IL13RA1 expression?

CRISPR/Cas9 knockout clarifies IL13RA1's functional impact.

How do siRNA knockdown experiments validate IL13RA1's involvement in specific pathways?

siRNA knockdown validates IL13RA1's pathway involvement.

Can you explain the role of co-immunoprecipitation in identifying IL13RA1 interaction partners?

Co-immunoprecipitation identifies IL13RA1 interactors.

How does immunofluorescence microscopy visualize IL13RA1 localization within cells?

Immunofluorescence microscopy visualizes intracellular IL13RA1 localization.

How does surface plasmon resonance (SPR) elucidate IL13RA1 binding kinetics?

SPR elucidates IL13RA1 binding kinetics with precision.

Can you describe how surface biotinylation studies reveal IL13RA1 membrane localization?

Surface biotinylation shows IL13RA1 membrane distribution.

How do fluorescence-based assays assess IL13RA1 internalization upon ligand binding?

Fluorescence assays monitor IL13RA1 internalization upon ligand binding.