Recombinant Rat Il1rn Protein, His-tagged

• Cat.No.: Il1rn-2299R
• Product Overview: Recombinant Rat Il1rn Protein (His27-Gln178) with a N-His tag was expressed in E. coli.

Specification

• Description: Enables interleukin-1 receptor binding activity. Involved in several processes, including cellular response to norepinephrine stimulus; chronic inflammatory response to antigenic stimulus; and fever generation. Located in extracellular space. Used to study adult respiratory distress syndrome; anti-basement membrane glomerulonephritis; hypertension; silicosis; and transient cerebral ischemia. Biomarker of encephalitis and pneumonia. Human ortholog(s) of this gene implicated in several diseases, including autoimmune disease (multiple); eye disease (multiple); kidney failure (multiple); lung disease (multiple); and vascular disease (multiple). Orthologous to human IL1RN (interleukin 1 receptor antagonist).
• Source: E. coli
• Species: Rat
• Tag: N-His
• Form: Freeze-dried powder
• Molecular Mass: Predicted Molecular Mass: 20.42 kDa
  Accurate Molecular Mass: 21 kDa
• Protein length: His27-Gln178
• Endotoxin: <1.0 EU per 1µg (determined by the LAL method).
• Purity: > 90%
• Applications: Positive Control; Immunogen; SDS-PAGE; WB.
• Stability: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 centigrade for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
• Storage: Avoid repeated freeze/thaw cycles. Store at 2-8 centigrade for one month. Aliquot and store at -80 centigrade for 12 months.
• Storage Buffer: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% SKL, 5% Trehalose and Proclin300.
• Reconstitution: Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Il1rn interleukin 1 receptor antagonist [ Rattus norvegicus (Norway rat) ]
• Official Symbol: Il1rn
• Synonyms: Il1rn; interleukin 1 receptor antagonist; IL-1ra; interleukin-1 receptor antagonist protein
  IL-1RN
  IL1 inhibitor
  IRAP
• Gene ID: 60582
• mRNA Refseq: NM_022194
• Protein Refseq: NP_071530
• UniProt ID: P25086