Active Recombinant Rat Il6 protein, His-tagged

• Cat.No.: Il6-481R
• Product Overview: Active Recombinant Rat Il6 protein(P20607)(25~211 aa), fused with N-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 25-211 aa
• Form: Lyophilized from sterile PBS, pH7.6, containing 5% trehalose.
• Bio-activity: Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot.
• Molecular Mass: 23.3kDa
• Purity: > 95% as determined by SDS-PAGE.
• Storage: Avoid repeated freeze/thaw cycles. Store at -20°C for 12 months. Aliquot and store at -80°C for 12 months.
• Reconstitution: Reconstitute in PBS (pH7.6) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

Gene Information

• Gene Name: Il6 interleukin 6 [ Rattus norvegicus ]
• Official Symbol: Il6
• Synonyms: IL6; interleukin 6; interleukin-6; IL-6; Interleukin 6 (interferon, beta 2); ILg6; Ifnb2
• Gene ID: 24498
• mRNA Refseq: NM_012589
• Protein Refseq: NP_036721