Active Recombinant Rat Il6 protein, His-tagged

• Cat.No.: Il6-481R
• Product Overview: Recombinant Rat Il6 protein(Phe25-Thr211), fused with C-terminal His tag, was expressed in HEK293.

Specification

• Species: Rat
• Source: HEK293
• Tag: His
• Protein Length: 25-211 aa
• Form: Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
• Bio-activity: 1. Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 30-123 pg/mL.
• Molecular Mass: 21.73 kDa
• Endotoxin: < 0.1 EU/μg of the protein by LAL method.
• Purity: ≥ 95 % as determined by SDS-PAGE.
• Storage: Store at -20°C.Store the lyophilized protein at -20°C to -80°C up to 1 year from the date of receipt.After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8°C for up to 1 week.
• Reconstitution: Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

Gene Information

• Gene Name: Il6 interleukin 6 [ Rattus norvegicus ]
• Official Symbol: Il6
• Synonyms: IL6; interleukin 6; interleukin-6; IL-6; Interleukin 6 (interferon, beta 2); ILg6; Ifnb2
• Gene ID: 24498
• mRNA Refseq: NM_012589
• Protein Refseq: NP_036721